ARG64834
anti-Annexin A1 antibody
anti-Annexin A1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat,Pig
Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes Annexin A1 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Pig |
| Predict Reactivity | Cow, Dog |
| Tested Application | IHC-P, WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Annexin A1 |
| Antigen Species | Human |
| Immunogen | CQAILDETKGDYEK |
| Conjugation | Un-conjugated |
| Alternate Names | ANX1; Calpactin II; Phospholipase A2 inhibitory protein; Chromobindin-9; LPC1; Annexin-1; Annexin A1; Lipocortin I; Annexin I; p35; Calpactin-2 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | Annexin I belongs to a family of Ca(2+)-dependent phospholipid binding proteins which have a molecular weight of approximately 35,000 to 40,000 and are preferentially located on the cytosolic face of the plasma membrane. Annexin I protein has an apparent relative molecular mass of 40 kDa, with phospholipase A2 inhibitory activity. Since phospholipase A2 is required for the biosynthesis of the potent mediators of inflammation, prostaglandins and leukotrienes, annexin I may have potential anti-inflammatory activity. [provided by RefSeq, Jul 2008] |
| Research Area | Signaling Transduction antibody |
| Calculated MW | 39 kDa |
| PTM | Phosphorylated by protein kinase C, EGFR and TRPM7 (PubMed:2457390, PubMed:15485879). Phosphorylated in response to EGF treatment (PubMed:2532504). Sumoylated. |
Images (6) Click the Picture to Zoom In
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ARG64834 anti-Annexin A1 antibody WB image
Western blot: A431 lysate (35 µg protein in RIPA buffer) stained with ARG64834 anti-Annexin A1 antibody at 1 µg/ml dilution.
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ARG64834 anti-Annexin A1 antibody WB image
Western blot: Rat Spleen lysate (35 µg protein in RIPA buffer) stained with ARG64834 anti-Annexin A1 antibody at 1 µg/ml dilution.
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ARG64834 anti-Annexin A1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human prostate tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64834 anti-Annexin A1 antibody at 4 µg/ml dilution followed by AP-staining.
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ARG64834 anti-Annexin A1 antibody WB image
Western blot: 35 µg of K562 (A) and NIH/3T3 (B) cell lysates (in RIPA buffer) stained with ARG64834 anti-Annexin A1 antibody at 1 µg/ml dilution and incubated at RT for 1 hour.
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ARG64834 anti-Annexin A1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human placenta tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64834 anti-Annexin A1 antibody at 4 µg/ml dilution followed by AP-staining.
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ARG64834 anti-Annexin A1 antibody WB image
Western blot: 35 µg of Pig spleen lysate (in RIPA buffer) stained with ARG64834 anti-Annexin A1 antibody at 1 µg/ml dilution and incubated at RT for 1 hour.
