ARG40206
anti-Annexin A1 antibody
anti-Annexin A1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Rat,Monkey
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Annexin A1 |
|---|---|
| Tested Reactivity | Hu, Rat, Mk |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Annexin A1 |
| Antigen Species | Human |
| Immunogen | Recombinant protein corresponding to A2-N346 of Human Annexin A1. |
| Conjugation | Un-conjugated |
| Alternate Names | ANX1; Calpactin II; Phospholipase A2 inhibitory protein; Chromobindin-9; LPC1; Annexin-1; Annexin A1; Lipocortin I; Annexin I; p35; Calpactin-2 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: By heat mediation. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 5% BSA. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ANXA1 |
| Gene Full Name | annexin A1 |
| Background | This gene encodes a membrane-localized protein that binds phospholipids. This protein inhibits phospholipase A2 and has anti-inflammatory activity. Loss of function or expression of this gene has been detected in multiple tumors. [provided by RefSeq, Dec 2014] |
| Function | Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. This protein regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity. [UniProt] |
| Cellular Localization | Endosome; Nucleus; Plasma membrane; Secreted. [UniProt] |
| Calculated MW | 39 kDa |
| PTM | Phosphorylated by protein kinase C, EGFR and TRPM7 (PubMed:2457390, PubMed:15485879). Phosphorylated in response to EGF treatment (PubMed:2532504). Sumoylated. [UniProt] |
Images (5) Click the Picture to Zoom In
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ARG40206 anti-Annexin A1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human tonsil stained with ARG40206 anti-Annexin A1 antibody.
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ARG40206 anti-Annexin A1 antibody WB image
Western blot: 0.5 ng of Recombinant Human Annexin A1 Protein stained with ARG40206 anti-Annexin A1 antibody at 0.5 µg/ml dilution.
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ARG40206 anti-Annexin A1 antibody WB image
Western blot: 30 µg of sample under reducing conditions. HeLa, K562, COS-7, A549, PC-3, U2OS, Rat spleen, Rat lung, Rat kidney and C6 whole cell lysates stained with ARG40206 anti-Annexin A1 antibody at 0.5 µg/ml dilution, overnight at 4°C.
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ARG40206 anti-Annexin A1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat lung stained with ARG40206 anti-Annexin A1 antibody.
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ARG40206 anti-Annexin A1 antibody WB image
Western blot: 40 µg of HepG2, MCF-7, U87, PANC, SMMC-7721, SW620, COLO320, A549 and Jurkat whole cell lysates stained with ARG40206 anti-Annexin A1 antibody at 0.5 µg/ml dilution.
