ARG54810
anti-Angiopoietin 2 antibody
anti-Angiopoietin 2 antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Cell Biology and Cellular Response antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Angiopoietin 2 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | FACS, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Angiopoietin 2 |
| Antigen Species | Human |
| Immunogen | KLH-conjugated synthetic peptide corresponding to aa. 404-432 (C-terminus) of Human Angiopoietin 2. |
| Conjugation | Un-conjugated |
| Alternate Names | ANG-2; Angiopoietin-2; ANG2; AGPT2 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | Mouse kidney |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A and immunogen peptide. |
| Buffer | PBS and 0.09% (W/V) Sodium azide |
| Preservative | 0.09% (W/V) Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ANGPT2 |
| Gene Full Name | angiopoietin 2 |
| Background | The protein encoded by this gene is an antagonist of angiopoietin 1 (ANGPT1) and endothelial TEK tyrosine kinase (TIE-2, TEK). The encoded protein disrupts the vascular remodeling ability of ANGPT1 and may induce endothelial cell apoptosis. Three transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Function | Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal. [UniProt] |
| Cellular Localization | Secreted. |
| Research Area | Cell Biology and Cellular Response antibody |
| Calculated MW | 57 kDa |
Images (3) Click the Picture to Zoom In
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ARG54810 anti-Angiopoietin 2 antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human uterus tissue stained with ARG54810 anti-Angiopoietin 2 antibody.
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ARG54810 anti-Angiopoietin 2 antibody WB image
Western blot: 35 µg of Mouse kidney lysate stained with ARG54810 anti-Angiopoietin 2 antibody.
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ARG54810 anti-Angiopoietin 2 antibody FACS image
Flow Cytometry: A549 cells stained with ARG54810 anti-Angiopoietin 2 antibody (right histogram) or without primary antibody control (left histogram), followed by incubation with FITC labelled secondary antibody.
