ARG41361

anti-Androgen Receptor antibody

anti-Androgen Receptor antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human

Overview

Product Description Rabbit Polyclonal antibody recognizes Androgen Receptor
Tested Reactivity Hu
Tested Application ICC/IF, IHC-P, IP, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Androgen Receptor
Antigen Species Human
Immunogen Synthetic peptide derived from Human Androgen Receptor.
Conjugation Un-conjugated
Alternate Names TFM; Dihydrotestosterone receptor; Androgen receptor; KD; AR8; HUMARA; NR3C4; AIS; SBMA; HYSP1; SMAX1; Nuclear receptor subfamily 3 group C member 4; DHTR

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50 - 1:100
IHC-P1:50 - 1:100
IP1:30
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size 100 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 367 Human AR

Swiss-port # P10275 Human Androgen receptor

Gene Symbol AR
Gene Full Name androgen receptor
Background Androgen Receptor is a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract from the normal 9-34 repeats to the pathogenic 38-62 repeats causes spinal bulbar muscular atrophy (SBMA, also known as Kennedy's disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2017]
Function Androgen Receptors are ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Transcription factor activity is modulated by bound coactivator and corepressor proteins like ZBTB7A that recruits NCOR1 and NCOR2 to the androgen response elements/ARE on target genes, negatively regulating androgen receptor signaling and androgen-induced cell proliferation (PubMed:20812024). Transcription activation is also down-regulated by NR0B2. Activated, but not phosphorylated, by HIPK3 and ZIPK/DAPK3.

Isoform 3 and isoform 4 lack the C-terminal ligand-binding domain and may therefore constitutively activate the transcription of a specific set of genes independently of steroid hormones. [UniProt]
Cellular Localization Nucleus. Cytoplasm. Note=Detected at the promoter of target genes (PubMed:25091737). Predominantly cytoplasmic in unligated form but translocates to the nucleus upon ligand-binding. Can also translocate to the nucleus in unligated form in the presence of RACK1. [UniProt]
Calculated MW 99 kDa
PTM Sumoylated on Lys-388 (major) and Lys-521. Ubiquitinated. Deubiquitinated by USP26. 'Lys-6' and 'Lys-27'-linked polyubiquitination by RNF6 modulates AR transcriptional activity and specificity.

Phosphorylated in prostate cancer cells in response to several growth factors including EGF. Phosphorylation is induced by c-Src kinase (CSK). Tyr-535 is one of the major phosphorylation sites and an increase in phosphorylation and Src kinase activity is associated with prostate cancer progression. Phosphorylation by TNK2 enhances the DNA-binding and transcriptional activity and may be responsible for androgen-independent progression of prostate cancer. Phosphorylation at Ser-83 by CDK9 regulates AR promoter selectivity and cell growth. Phosphorylation by PAK6 leads to AR-mediated transcription inhibition.

Palmitoylated by ZDHHC7 and ZDHHC21. Palmitoylation is required for plasma membrane targeting and for rapid intracellular signaling via ERK and AKT kinases and cAMP generation. [UniProt]

Images (3) Click the Picture to Zoom In

  • ARG41361 anti-Androgen Receptor antibody ICC/IF image

    Immunofluorescence: LNCaP cells stained with ARG41361 anti-Androgen Receptor antibody.

  • ARG41361 anti-Androgen Receptor antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human prostate carcinoma tissue stained with ARG41361 anti-Androgen Receptor antibody.

  • ARG41361 anti-Androgen Receptor antibody WB image

    Western blot: T47D cell lysate stained with ARG41361 anti-Androgen Receptor antibody.