ARG45652

anti-Aly / Ref antibody

anti-Aly / Ref antibody for Flow cytometry,ICC/IF,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes Aly / Ref
Tested Reactivity Hu, Ms, Rat
Tested Application FACS, ICC/IF, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Aly / Ref
Antigen Species Human
Immunogen Recombinant protein containing to human Aly / Ref.
Conjugation Un-conjugated
Alternate Names ALYREF; Aly/REF Export Factor; ALY; BEF; THO Complex Subunit 4; ALY/REF; THOC4; REF; Transcriptional Coactivator Aly/REF; BZIP-Enhancing Factor BEF; Ally Of AML-1 And LEF-1; THO Complex 4; Tho4; BZIP Enhancing Factor

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 3 µg/10^6 cells
ICC/IF5 μg/ml
WB0.25-0.5 μg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size 35 kDa

Properties

Form Liquid
Purification Affinity purified
Buffer 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose.
Stabilizer 4% Trehalose
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 10189 Human ALYREF

GeneID: 21681 Mouse ALYREF

Swiss-port # O08583 Mouse THO complex subunit 4

Swiss-port # Q86V81 Human THO complex subunit 4

Gene Symbol ALYREF
Gene Full Name Aly/REF Export Factor
Background The protein encoded by this gene is a heat stable, nuclear protein and functions as a molecular chaperone. It is thought to regulate dimerization, DNA binding, and transcriptional activity of basic region-leucine zipper (bZIP) proteins. [provided by RefSeq, Jul 2008]
Function Functions as an mRNA export adapter; component of the transcription/export (TREX) complex which is thought to couple mRNA transcription, processing and nuclear export, and specifically associates with spliced mRNA and not with unspliced pre-mRNA. [UniProt]
Cellular Localization Cytoplasm; Nucleus; Spliceosome. [UniProt]
Calculated MW 27 kDa
PTM Acetylation; Citrullination; Methylation; Phosphoprotein. [UniProt]

Images (5) Click the Picture to Zoom In

  • ARG45652 anti-Aly / Ref antibody ICC/IF image

    Immunofluorescence: A431 stained with ARG45652 anti-Aly / Ref antibody at 5 μg/ml dilution.

  • ARG45652 anti-Aly / Ref antibody WB image

    Western blot: Human placenta and PC-3 stained with ARG45652 anti-Aly / Ref antibody at 0.5 μg/ml dilution.

  • ARG45652 anti-Aly / Ref antibody FACS image

    Flow Cytometry: SiHa stained with ARG45652 anti-Aly / Ref antibody at 1 µg/10^6 cells dilution.

  • ARG45652 anti-Aly / Ref antibody WB image

    Western blot: Rat brain stained with ARG45652 anti-Aly / Ref antibody at 0.5 μg/ml dilution.

  • ARG45652 anti-Aly / Ref antibody WB image

    Western blot: RAW264.7 stained with ARG45652 anti-Aly / Ref antibody at 0.5 μg/ml dilution.