ARG10694
anti-Aldolase C antibody [1A1]
anti-Aldolase C antibody [1A1] for ICC/IF,IHC-Frozen sections,Western blot and Human,Mouse,Rat,Cow,Horse,Pig
Overview
| Product Description | Mouse Monoclonal antibody [1A1] recognizes Aldolase C |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Cow, Hrs, Pig |
| Predict Reactivity | Chk |
| Tested Application | ICC/IF, IHC-Fr, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 1A1 |
| Isotype | IgG1 |
| Target Name | Aldolase C |
| Immunogen | C-terminal sequence KYEGSGEDGGAAAQSLYIANHAY |
| Conjugation | Un-conjugated |
| Alternate Names | EC 4.1.2.13; Brain-type aldolase; Fructose-bisphosphate aldolase C; ALDC |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification. |
| Buffer | PBS and 50% Glycerol. |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ALDOC |
| Gene Full Name | aldolase C, fructose-bisphosphate |
| Background | This gene encodes a member of the class I fructose-biphosphate aldolase gene family. Expressed specifically in the hippocampus and Purkinje cells of the brain, the encoded protein is a glycolytic enzyme that catalyzes the reversible aldol cleavage of fructose-1,6-biphosphate and fructose 1-phosphate to dihydroxyacetone phosphate and either glyceraldehyde-3-phosphate or glyceraldehyde, respectively. [provided by RefSeq, Jul 2008] |
| Calculated MW | 39 kDa |
Images (3) Click the Picture to Zoom In
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ARG10694 anti-Aldolase C antibody [1A1] ICC/IF image
Immunocytochemistry: Rat mixed neuron / glial cultures stained with ARG10694 anti-Aldolase C antibody [1A1] (green) and co-stained with rabbit antibody to NeuN/FOX3 antibody (RPCA-FOX3, red). MCA-1A1 antibody reveals strong cytoplasmic staining in astrocytes, while Rabbit Fox3/NeuN antibody shows nuclear and distal cytoplasmic staining in neuron cells and is complete absence of astrocytes. Blue is a DNA stain.
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ARG10694 anti-Aldolase C antibody [1A1] WB image
Western blot: Crude Rat brain lysates stained with ARG10694 anti-Aldolase C antibody [1A1]. Other studies show that this antibody is completely specific for Aldolase C, and does not react with recombinant Human Aldolase A or B.
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ARG10694 anti-Aldolase C antibody [1A1] WB image
Western blot: Recombinant Human Aldolase A, B and C with three monoclonal antibodies as indicated showing binding to all three gene products. Lane labelled S show molecular weight standards, while lanes A, B and C contain recombinant full length His-tagged Human Aldolase A, B and C respectively. The epitope for ARG10694 anti-Aldolase C antibody [1A1] is within the C-terminal peptide of the molecule which is less conserved between the three Aldolases. Clone 4A9 binds to the N-terminal peptide and also is specific for Aldolase C, while clone E9 binds to the less conserved core of the molecule and recognizes all three Aldolase C gene products.
