ARG43631
anti-AlaRS / Alanyl tRNA Synthetase antibody
anti-AlaRS / Alanyl tRNA Synthetase antibody for Flow cytometry,ICC/IF,Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes AlaRS / Alanyl tRNA Synthetase |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | AlaRS / Alanyl tRNA Synthetase |
| Antigen Species | Human |
| Immunogen | Recombinant fragment corresponding to a.a. R729-N968 of Human Alanyl-tRNA Synthetase. |
| Conjugation | Un-conjugated |
| Protein Full Name | Alanine--tRNA ligase, cytoplasmic |
| Alternate Names | Renal carcinoma antigen NY-REN-42; CMT2N; EIEE29; AlaRS; EC 6.1.1.7; Alanine--tRNA ligase, cytoplasmic; Alanyl-tRNA synthetase |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | HeLa; K562; HepG2 cells | ||||||||
| Observed Size | 110-120 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.9% NaCl, 0.2% Na2HPO4 and 4% Trehalose. |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | AARS |
| Gene Full Name | alanyl-tRNA synthetase |
| Background | The human alanyl-tRNA synthetase (AARS) belongs to a family of tRNA synthases, of the class II enzymes. Class II tRNA synthases evolved early in evolution and are highly conserved. This is reflected by the fact that 498 of the 968-residue polypeptide human AARS shares 41% identity witht the E.coli protein. tRNA synthases are the enzymes that interpret the RNA code and attach specific aminoacids to the tRNAs that contain the cognate trinucleotide anticodons. They consist of a catalytic domain which interacts with the amino acid acceptor-T psi C helix of the tRNA, and a second domain which interacts with the rest of the tRNA structure. [provided by RefSeq, Jul 2008] |
| Function | Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged tRNA(Ala) via its editing domain. [UniProt] |
| Cellular Localization | Cytoplasm [UniProt] |
| Calculated MW | 107 kDa |
| PTM | ISGylated. [UniProt] |
Images (3) Click the Picture to Zoom In
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ARG43631 anti-AlaRS / Alanyl tRNA Synthetase antibody ICC/IF image
Immunofluorescence: MCF-7 cells were stained with ARG43631 anti-AlaRS / Alanyl tRNA Synthetase antibody at 1:100 dilution, 30 minutes at 37°C.
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ARG43631 anti-AlaRS / Alanyl tRNA Synthetase antibody WB image
Western blot: HeLa cell lysates stained with ARG43631 anti-AlaRS / Alanyl tRNA Synthetase antibody, at 1:1000 dilution.
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ARG43631 anti-AlaRS / Alanyl tRNA Synthetase antibody FACS image
Flow Cytometry: K562 cells were stained ARG43631 anti-AlaRS / Alanyl tRNA Synthetase antibody in 1x PBS/1% BSA for 30 min at RT.
