ARG63666
anti-Akt 3 antibody
anti-Akt 3 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Cancer antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes Akt 3 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Predict Reactivity | Cow, Dog |
| Tested Application | IHC-P, WB |
| Specificity | This antibody is expected to recognise an epitope corresponding to aa 119-133 of both isoforms of human AKT3 protein. This antibody does not cross-react with human AKT1/2. Reported variants represent identical protein (NP_859029.1; NP_001193658.1). |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Akt 3 |
| Antigen Species | Human |
| Immunogen | CSPTSQIDNIGEEEM |
| Conjugation | Un-conjugated |
| Alternate Names | Protein kinase Akt-3; PKB-GAMMA; PKB gamma; RAC-gamma serine/threonine-protein kinase; STK-2; PRKBG; RAC-gamma; MPPH2; MPPH; Protein kinase B gamma; EC 2.7.11.1; PKBG; RAC-PK-gamma |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | The protein encoded by this gene is a member of the AKT, also called PKB, serine/threonine protein kinase family. AKT kinases are known to be regulators of cell signaling in response to insulin and growth factors. They are involved in a wide variety of biological processes including cell proliferation, differentiation, apoptosis, tumorigenesis, as well as glycogen synthesis and glucose uptake. This kinase has been shown to be stimulated by platelet-derived growth factor (PDGF), insulin, and insulin-like growth factor 1 (IGF1). Alternatively splice transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008] |
| Research Area | Cancer antibody; Signaling Transduction antibody |
| Calculated MW | 56 kDa |
| PTM | Phosphorylation on Thr-305 and Ser-472 is required for full activity. Ubiquitinated. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome. O-GlcNAcylation at Thr-302 and Thr-309 inhibits activating phosphorylation at Thr-305 via disrupting the interaction between AKT and PDK1. |
Images (4) Click the Picture to Zoom In
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ARG63666 anti-Akt 3 antibody WB image
Western blot: HepG2 (A), Jurkat (B), Mouse Brain (C) and Rat Brain (D) lysates (35 µg protein in RIPA buffer) stained with ARG63666 anti-Akt 3 antibody at 1 µg/ml dilution.
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ARG63666 anti-Akt 3 antibody WB image
Western blot: 1). AKT3 (RC224750) expressing plasmid transfected; 2) Mock transfection HEK293 cell lysate standed with ARG63666 anti-Akt 3 antibody.
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ARG63666 anti-Akt 3 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human prostate tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63666 anti-Akt 3 antibody at 5 µg/ml dilution followed by AP-staining.
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ARG63666 anti-Akt 3 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human heart tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63666 anti-Akt 3 antibody at 5 µg/ml dilution followed by AP-staining.
