ARG62353
anti-Adenovirus Fiber monomer and trimer antibody [4D2]
anti-Adenovirus Fiber monomer and trimer antibody [4D2] for Blocking,Flow cytometry,ICC/IF,Western blot and Virus
Microbiology and Infectious Disease antibody
Overview
| Product Description | Mouse Monoclonal antibody [4D2] recognizes Adenovirus Fiber monomer and trimer |
|---|---|
| Tested Reactivity | Virus |
| Tested Application | BL, FACS, ICC/IF, WB |
| Specificity | ARG62353 recognizes Ad2, Ad3, and Ad5 monomers in boiled samples and trimers in un-boiled (DTT, 2-ME free) samples. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 4D2 |
| Isotype | IgG2a |
| Target Name | Adenovirus Fiber monomer and trimer |
| Antigen Species | Virus |
| Immunogen | UV irradiated Ad2 virus |
| Epitope | N-terminal |
| Conjugation | Un-conjugated |
Application Instructions
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
|---|---|
| Positive Control | Adenovirus infected cells and tissues |
Properties
| Form | Liquid |
|---|---|
| Purification | Protein A purified |
| Buffer | 10mM PBS (pH 7.4), 0.2% BSA and 0.09% Sodium azide |
| Preservative | 0.09% Sodium azide |
| Stabilizer | 0.2% BSA |
| Concentration | 0.2 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | Adenovirus capsid proteins are synthesized in the cytoplasm and transported to the nucleus for assembly into the virus particles. The three major capsid proteins (hexon, penton base, and fiber) are synthesized late in infection. Fiber plays a crucial role in adenovirus infection by attaching the virus to a specific receptor on the cell surface. Ad2 and Ad5 fibers are proposed to consist of three domains: an N terminal tail that interacts with penton base, a shaft composed of 22 repeats of a 15 amino acid segment that forms beta-sheet and beta-bends. A knob at the C terminus contains the type specific antigen and is responsible for binding to the cell surface receptor. It is shown that the fiber of Ad2 is most likely a trimer when found on the viron. |
| Cellular Localization | Nuclear |
| Research Area | Microbiology and Infectious Disease antibody |
| Calculated MW | 62 kDa |
Clone References
Immunization with Hexon modified adenoviral vectors integrated with gp83 epitope provides protection against Trypanosoma cruzi infection.
WB /
An adenovirus vector incorporating carbohydrate binding domains utilizes glycans for gene transfer.
WB / Virus
Modeling adenovirus latency in human lymphocyte cell lines.
FACS /
INGN 007, an oncolytic adenovirus vector, replicates in Syrian hamsters but not mice: comparison of biodistribution studies.
IHC / Hamster
Transthyretin-driven oncolytic adenovirus suppresses tumor growth in orthotopic and ascites models of hepatocellular carcinoma.
WB, ICC/IF /
A human adenoviral vector with a chimeric fiber from canine adenovirus type 1 results in novel expanded tropism for cancer gene therapy.
IHC / Human
