ARG62666
anti-Acrosin antibody [ACR-2]
anti-Acrosin antibody [ACR-2] for ICC/IF,Western blot and Pig
Cell Biology and Cellular Response antibody
Overview
| Product Description | Mouse Monoclonal antibody [ACR-2] recognizes Acrosin |
|---|---|
| Tested Reactivity | Pig |
| Species Does Not React With | Hu, Bov, Dog |
| Tested Application | ICC/IF, WB |
| Specificity | The clone ACR-2 reacts with various forms of porcine acrosin (55, 53, 45 and 35 kDa), a typical serine proteinase with trypsin-like specificity. Acrosin is stored in the acrosome of undamaged spermatozoa. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | ACR-2 |
| Isotype | IgG1 |
| Target Name | Acrosin |
| Immunogen | Acid extracts of boar spermatozoa were subjected to hydrophobic chromatography and the pooled fraction with reactivity to N-alpha benzoylarginine-4-nitroanilide was used for immunization. |
| Conjugation | Un-conjugated |
| Alternate Names | Acrosin; EC 3.4.21.10 |
Application Instructions
| Application Suggestion |
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| Application Note | FACS: Described in Ded et al., 2010 ICC/IF: Membrane permeabilization (acetone) is essential. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from ascites by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ACR |
| Gene Full Name | acrosin |
| Background | Acrosin is the major proteinase present in the acrosome of mature spermatozoa. It is a typical serine proteinase with trypsin-like specificity. It is stored in the acrosome in its precursor form, proacrosin. The active enzyme functions in the lysis of the zona pellucida, thus facilitating penetration of the sperm through the innermost glycoprotein layers of the ovum. The mRNA for proacrosin is synthesized only in the postmeiotic stages of spermatogenesis. In humans proacrosin first appears in the haploid spermatids. [provided by RefSeq, Jul 2008] |
| Function | Acrosin is the major protease of mammalian spermatozoa. It is a serine protease of trypsin-like cleavage specificity, it is synthesized in a zymogen form, proacrosin and stored in the acrosome. [UniProt] |
| Research Area | Cell Biology and Cellular Response antibody |
| Calculated MW | 46 kDa |
Images (1) Click the Picture to Zoom In
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ARG62666 anti-Acrosin antibody [ACR-2] ICC/IF image
Immunofluorescence: Capacitated boar sperm. Acrosome visualized using ARG62666 anti-Acrosin antibody [ACR-2] (green), DAPI (blue) for nuclear staining.
Clone References
Relationship between molecular conversions of acrosin and the progression of exocytosis in the calcium ionophore-induced acrosome reaction.
Protein-protein interactions controlling acrosin release and solubilization during the boar sperm acrosome reaction.
Monoclonal antibodies against boar acrosomal antigens labelling undamaged acrosomes of spermatozoa in immunofluorescence test.
Mechanism of maturation and nature of carbohydrate chains of boar sperm acrosin.
