ARG57057
anti-Acid Phosphatase antibody [5C11]
anti-Acid Phosphatase antibody [5C11] for Western blot and Human
Overview
| Product Description | Mouse Monoclonal antibody [5C11] recognizes Acid Phosphatase |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 5C11 |
| Isotype | IgG1, kappa |
| Target Name | Acid Phosphatase |
| Antigen Species | Human |
| Immunogen | Recombinant fragment around aa. 1-158 of Human Acid Phosphatase. |
| Conjugation | Un-conjugated |
| Alternate Names | Low molecular weight phosphotyrosine protein phosphatase; LMW-PTP; HAAP; Red cell acid phosphatase 1; EC 3.1.3.2; Low molecular weight cytosolic acid phosphatase; Adipocyte acid phosphatase; EC 3.1.3.48; LMW-PTPase |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 10% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P24666 Human Low molecular weight phosphotyrosine protein phosphatase |
|---|---|
| Gene Symbol | ACP1 |
| Gene Full Name | acid phosphatase 1, soluble |
| Background | The product of this gene belongs to the phosphotyrosine protein phosphatase family of proteins. It functions as an acid phosphatase and a protein tyrosine phosphatase by hydrolyzing protein tyrosine phosphate to protein tyrosine and orthophosphate. This enzyme also hydrolyzes orthophosphoric monoesters to alcohol and orthophosphate. This gene is genetically polymorphic, and three common alleles segregating at the corresponding locus give rise to six phenotypes. Each allele appears to encode at least two electrophoretically different isozymes, Bf and Bs, which are produced in allele-specific ratios. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Aug 2008] |
| Function | Acts on tyrosine phosphorylated proteins, low-MW aryl phosphates and natural and synthetic acyl phosphates. Isoform 3 does not possess phosphatase activity. [UniProt] |
| Calculated MW | 18 kDa |
Images (1) Click the Picture to Zoom In
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ARG57057 anti-Acid Phosphatase antibody [5C11] WB image
Western blot: 40 µg of 1) HeLa cell lysate, 2) Jurkat cell lysate, 3) TF-1 cell lysate, 4) NIH/3T3 cell lysate, 5) HepG2 cell lysate stained with ARG57057 anti-Acid Phosphatase antibody [5C11] at 1:1000.
