ARG43600

anti-ATP8 antibody

anti-ATP8 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes ATP8
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name ATP8
Antigen Species Human
Immunogen Recombinant protein corresponding to a sequence of human ATP8.
Conjugation Un-conjugated
Protein Full Name ATP synthase protein 8
Alternate Names ATPase8; MTATP8; ATP8TP synthase protein 8; A6L; F-ATPase subunit 8; MT-ATP8; ATP8; ATPASE8; mitochondrially encoded ATP synthase 8

Application Instructions

Predict Reactivity Note Mouse, Rat
Application Suggestion
Tested Application Dilution
ICC/IF1:50 - 1:200
IHC-P1:50 - 1:200
WB1:50 - 1:100
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control HeLa, JurKat
Observed Size ~ 9 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 17706 Mouse MT-ATP8

GeneID: 26196 Rat MT-ATP8

GeneID: 4509 Human MT-ATP8

Gene Symbol MT-ATP8
Gene Full Name mitochondrially encoded ATP synthase 8
Function Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F0 domain. Minor subunit located with subunit a in the membrane (By similarity). [UniProt]
Cellular Localization CF(0), Membrane, Mitochondrion
Calculated MW 7.9 kDa
PTM Acetylation

Images (2) Click the Picture to Zoom In

  • ARG43600 anti-ATP8 antibody ICC/IF image

    Immunofluorescence: HeLa cells stained with ARG43600 anti-ATP8 antibody at 1:100 dilution.

  • ARG43600 anti-ATP8 antibody WB image

    Western blot: JurKat cell lysate stained with ARG43600 anti-ATP8 antibody at 1:1000 dilution.