Overview

Product Description	Rabbit Polyclonal antibody recognizes ATP5O
Tested Reactivity	Hu, Ms, Rat
Tested Application	ICC/IF, IHC-P, WB
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Target Name	ATP5O
Antigen Species	Human
Immunogen	Recombinant Protein of Human ATP5O.
Conjugation	Un-conjugated
Alternate Names	Oligomycin sensitivity conferral protein; ATP synthase subunit O, mitochondrial; ATPO; HMC08D05; OSCP

Application Instructions

Application Suggestion	Tested Application Dilution ICC/IF 1:50 - 1:200 IHC-P 1:50 - 1:200 WB 1:500 - 1:2000
Application Note	* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control	MCF7

Properties

Form	Liquid
Purification	Affinity purification with immunogen.
Buffer	PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative	0.02% Sodium azide
Stabilizer	50% Glycerol
Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 192241 Rat ATP5O GeneID: 28080 Mouse ATP5O GeneID: 539 Human ATP5O Swiss-port # P48047 Human ATP synthase subunit O, mitochondrial Swiss-port # Q06647 Rat ATP synthase subunit O, mitochondrial Swiss-port # Q9DB20 Mouse ATP synthase subunit O, mitochondrial More
Gene Symbol	ATP5O
Gene Full Name	ATP synthase, H+ transporting, mitochondrial F1 complex, O subunit
Background	The protein encoded by this gene is a component of the F-type ATPase found in the mitochondrial matrix. F-type ATPases are composed of a catalytic core and a membrane proton channel. The encoded protein appears to be part of the connector linking these two components and may be involved in transmission of conformational changes or proton conductance. [provided by RefSeq, Jul 2008]
Function	Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements. [UniProt]
Calculated MW	23 kDa
PTM	Acetylation at Lys-162 decreases ATP production. Deacetylated by SIRT3.

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