ARG44410

anti-ATP5F1D antibody

anti-ATP5F1D antibody for IHC-Formalin-fixed paraffin-embedded sections,ICC/IF,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes ATP5F1D
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Target Name ATP5F1D
Antigen Species Human
Immunogen Human ATP5F1D recombinant protein (aa. sequence: R8-E168).
Conjugation Un-conjugated
Alternate Names ATP5F1D; ATP Synthase F1 Subunit Delta; ATP5D; ATP Synthase, H+ Transporting, Mitochondrial F1 Complex, Delta Subunit; ATP Synthase Subunit Delta, Mitochondrial; F-ATPase Delta Subunit; Mitochondrial ATP Synthase Complex Delta-Subunit Precusor; Mitochondrial ATP Synthase, Delta Subunit; MC5DN5

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF5 µg/ml
IHC-P1-2 µg/ml
WB0.25-0.5 µg/ml
Application Note The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 4% Trehalose.
Preservative 0.05% Sodium azide
Stabilizer 4% Trehalose
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.

Bioinformation

Database Links

GeneID: 245965 Rat ATP5D

GeneID: 513 Human ATP5D

GeneID: 66043 Mouse ATP5D

Gene Symbol ATP5F1D
Gene Full Name ATP Synthase F1 Subunit Delta
Background This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the delta subunit of the catalytic core. Alternatively spliced transcript variants encoding the same isoform have been identifie
Function Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain.
Cellular Localization CF(1), Membrane, Mitochondrion, Mitochondrion inner membrane
Calculated MW 17 kDa
PTM Acetylation

Images (6) Click the Picture to Zoom In

  • ARG44410 anti-ATP5F1D antibody IHC-P image

    Immunohistochemistry: Human colorectal adenocarcinoma stained with ARG44410 anti-ATP5F1D antibody at 2 μg/mL dilution.

  • ARG44410 anti-ATP5F1D antibody ICC/IF image

    Immunofluorescence: HeLa stained with ARG44410 anti-ATP5F1D antibody at 5 μg/mL dilution.

  • ARG44410 anti-ATP5F1D antibody WB image

    Western blot: A549, HepG2 and MCF-7 stained with ARG44410 anti-ATP5F1D antibody at 0.5 μg/mL dilution.

  • ARG44410 anti-ATP5F1D antibody IHC-P image

    Immunohistochemistry: Rat heart stained with ARG44410 anti-ATP5F1D antibody at 2 μg/mL dilution.

  • ARG44410 anti-ATP5F1D antibody WB image

    Western blot: Rat liver stained with ARG44410 anti-ATP5F1D antibody at 0.5 μg/mL dilution.

  • ARG44410 anti-ATP5F1D antibody WB image

    Western blot: Mouse liver stained with ARG44410 anti-ATP5F1D antibody at 0.5 μg/mL dilution.