ARG44410
anti-ATP5F1D antibody
anti-ATP5F1D antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes ATP5F1D |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | ATP5F1D |
| Antigen Species | Human |
| Immunogen | Human ATP5F1D recombinant protein (aa. sequence: R8-E168). |
| Conjugation | Un-conjugated |
| Alternate Names | ATP5F1D; ATP Synthase F1 Subunit Delta; ATP5D; ATP Synthase, H+ Transporting, Mitochondrial F1 Complex, Delta Subunit; ATP Synthase Subunit Delta, Mitochondrial; F-ATPase Delta Subunit; Mitochondrial ATP Synthase Complex Delta-Subunit Precusor; Mitochondrial ATP Synthase, Delta Subunit; MC5DN5 |
Application Instructions
| Application Suggestion |
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| Application Note | The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 4% Trehalose. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ATP5F1D |
| Gene Full Name | ATP Synthase F1 Subunit Delta |
| Background | This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the delta subunit of the catalytic core. Alternatively spliced transcript variants encoding the same isoform have been identifie |
| Function | Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. |
| Cellular Localization | CF(1), Membrane, Mitochondrion, Mitochondrion inner membrane |
| Calculated MW | 17 kDa |
| PTM | Acetylation |
Images (6) Click the Picture to Zoom In
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ARG44410 anti-ATP5F1D antibody IHC-P image
Immunohistochemistry: Human colorectal adenocarcinoma stained with ARG44410 anti-ATP5F1D antibody at 2 μg/mL dilution.
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ARG44410 anti-ATP5F1D antibody ICC/IF image
Immunofluorescence: HeLa stained with ARG44410 anti-ATP5F1D antibody at 5 μg/mL dilution.
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ARG44410 anti-ATP5F1D antibody WB image
Western blot: A549, HepG2 and MCF-7 stained with ARG44410 anti-ATP5F1D antibody at 0.5 μg/mL dilution.
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ARG44410 anti-ATP5F1D antibody IHC-P image
Immunohistochemistry: Rat heart stained with ARG44410 anti-ATP5F1D antibody at 2 μg/mL dilution.
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ARG44410 anti-ATP5F1D antibody WB image
Western blot: Rat liver stained with ARG44410 anti-ATP5F1D antibody at 0.5 μg/mL dilution.
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ARG44410 anti-ATP5F1D antibody WB image
Western blot: Mouse liver stained with ARG44410 anti-ATP5F1D antibody at 0.5 μg/mL dilution.
