ARG55557
anti-ATP1B2 / Na+ K+ ATPase beta 2 antibody
anti-ATP1B2 / Na+ K+ ATPase beta 2 antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Signaling Transduction antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes ATP1B2 / Na+ K+ ATPase beta 2 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Predict Reactivity | Bov, Rb |
| Tested Application | FACS, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | ATP1B2 / Na+ K+ ATPase beta 2 |
| Antigen Species | Human |
| Immunogen | KLH-conjugated synthetic peptide corresponding to aa. 115-141 (Center) of Human Na+ K+ ATPase beta 2. |
| Conjugation | Un-conjugated |
| Alternate Names | Sodium/potassium-dependent ATPase subunit beta-2; Sodium/potassium-transporting ATPase subunit beta-2; AMOG; Adhesion molecule in glia; adhesion molecule on glia; AT1B2; ATPB2; ATPB2S; Na+ K+ ATPase beta 2; Na K ATPase beta 2; sodium potassium ATPase beta 2; ATPase Na+ K+ beta 2; ATPase Na K beta 2; ATPase sodium potassium beta 2 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | Human brain |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A and immunogen peptide. |
| Buffer | PBS and 0.09% (W/V) Sodium azide |
| Preservative | 0.09% (W/V) Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P14231 Mouse Sodium/potassium-transporting ATPase subunit beta-2 Swiss-port # P14415 Human Sodium/potassium-transporting ATPase subunit beta-2 |
|---|---|
| Gene Symbol | ATP1B2 |
| Gene Full Name | ATPase, Na+/K+ transporting, beta 2 polypeptide |
| Background | The protein encoded by this gene belongs to the family of Na+/K+ and H+/K+ ATPases beta chain proteins, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. The glycoprotein subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes a beta 2 subunit. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2014] |
| Function | This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The exact function of the beta-2 subunit is not known. Mediates cell adhesion of neurons and astrocytes, and promotes neurite outgrowth. [UniProt] |
| Cellular Localization | Cell membrane; Single-pass type II membrane protein |
| Research Area | Signaling Transduction antibody |
| Calculated MW | 33 kDa |
Images (3) Click the Picture to Zoom In
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ARG55557 anti-ATP1B2 / Na+ K+ ATPase beta 2 antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human hepatocarcinoma stained with ARG55557 anti-ATP1B2 / Na+ K+ ATPase beta 2 antibody.
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ARG55557 anti-ATP1B2 / Na+ K+ ATPase beta 2 antibody WB image
Western blot: Human brain lysate stained with ARG55557 anti-ATP1B2 / Na+ K+ ATPase beta 2 antibody at 1:1000 dilution.
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ARG55557 anti-ATP1B2 / Na+ K+ ATPase beta 2 antibody FACS image
Flow Cytometry: K562 cells stained with ARG55557 anti-ATP1B2 / Na+ K+ ATPase beta 2 antibody (bottom histogram) or without primary antibody control (top histogram), followed by incubation with FITC labelled secondary antibody.
