ARG58290
anti-ATP1A4 / Na+ K+ ATPase alpha 4 antibody
anti-ATP1A4 / Na+ K+ ATPase alpha 4 antibody for Flow cytometry,Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes ATP1A4 / Na+ K+ ATPase alpha 4 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | ATP1A4 / Na+ K+ ATPase alpha 4 |
| Antigen Species | Human |
| Immunogen | KLH-conjugated synthetic peptide corresponding to aa. 12-46 of Human ATP1A4. |
| Conjugation | Un-conjugated |
| Alternate Names | Sodium pump subunit alpha-4; Sodium/potassium-transporting ATPase subunit alpha-4; ATP1AL2; EC 3.6.3.9; ATP1A1; Na+ K+ ATPase alpha 4; Na K ATPase alpha 4; sodium potassium ATPase alpha 4; ATPase Na+ K+ alpha 4; ATPase Na K alpha 4; ATPase sodium potassium alpha 4 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | Human brain |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A and immunogen peptide. |
| Buffer | PBS and 0.09% (W/V) Sodium azide. |
| Preservative | 0.09% (W/V) Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q13733 Human Sodium/potassium-transporting ATPase subunit alpha-4 |
|---|---|
| Gene Symbol | ATP1A4 |
| Gene Full Name | ATPase, Na+/K+ transporting, alpha 4 polypeptide |
| Background | The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 4 subunit. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008] |
| Function | This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients. Plays a role in sperm motility. [UniProt] |
| Calculated MW | 114 kDa |
Images (2) Click the Picture to Zoom In
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ARG58290 anti-ATP1A4 / Na+ K+ ATPase alpha 4 antibody WB image
Western blot: 20 µg of Human brain lysate stained with ARG58290 anti-ATP1A4 / Na+ K+ ATPase alpha 4 antibody at 1:2000 dilution.
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ARG58290 anti-ATP1A4 / Na+ K+ ATPase alpha 4 antibody FACS image
Flow Cytometry: HeLa cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 2% BSA to block non-specific protein-protein interactions and stained with ARG58290 anti-ATP1A4 / Na+ K+ ATPase alpha 4 antibody (right histogram) at 1:25 dilution for 60 min at 37°C, followed by incubation with DyLight® 488 labelled secondary antibody. Isotype control antibody (left histogram) was Rabbit IgG1 (1 µg/10^6 cells) used under the same conditions. Acquisition of >10000 events was performed.
