ARG66327
anti-ATP1A1 / Na+ K+ ATPase alpha 1 antibody [SQab1875]
anti-ATP1A1 / Na+ K+ ATPase alpha 1 antibody [SQab1875] for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat
Overview
| Product Description | Recombinant Rabbit Monoclonal antibody [SQab1875] recognizes ATP1A1 / Na+ K+ ATPase alpha 1 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, IHC-P, IP, WB |
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | SQab1875 |
| Isotype | IgG |
| Target Name | ATP1A1 / Na+ K+ ATPase alpha 1 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide around the N-terminus of Human ATP1A1 / Na+ K+ ATPase alpha 1. |
| Conjugation | Un-conjugated |
| Alternate Names | Sodium pump subunit alpha-1; Sodium/potassium-transporting ATPase subunit alpha-1; EC 3.6.3.9; Na+ K+ ATPase alpha 1; Na K ATPase alpha 1; sodium potassium ATPase alpha 1; ATPase Na+ K+ alpha 1; ATPase Na K alpha 1; ATPase sodium potassium alpha 1 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA. |
| Preservative | 0.01% Sodium azide |
| Stabilizer | 40% Glycerol and 0.05% BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ATP1A1 |
| Gene Full Name | ATPase, Na+/K+ transporting, alpha 1 polypeptide |
| Background | ATP1A protein belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009] |
| Function | ATP1A is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients. [UniProt] |
| Highlight | Related products: Na K ATPase antibodies; Anti-Rabbit IgG secondary antibodies; Related news: Gene therapy for retinitis pigmentosa (RP) |
| Calculated MW | 113 kDa |
| PTM | Phosphorylation on Tyr-10 modulates pumping activity. Phosphorylation of Ser-943 by PKA modulates the response of ATP1A1 to PKC. Dephosphorylation by protein phosphatase 2A (PP2A) following increases in intracellular sodium, leading to increase catalytic activity (By similarity). [UniProt] |
Images (5) Click the Picture to Zoom In
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ARG66327 anti-ATP1A1 / Na+ K+ ATPase alpha 1 antibody [SQab1875] FACS image
Flow Cytometry: HeLa cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were stained with ARG66327 anti-ATP1A1 / Na+ K+ ATPase alpha 1 antibody [SQab1875] (red) at 1:200 dilution in 1x PBS/1% BSA for 30 min at RT, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (black) was used as a control.
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ARG66327 anti-ATP1A1 / Na+ K+ ATPase alpha 1 antibody [SQab1875] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded endometrium tissue stained with ARG66327 anti-ATP1A1 / Na+ K+ ATPase alpha 1 antibody [SQab1875] at 1:10,000 dilution. Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0.
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ARG66327 anti-ATP1A1 / Na+ K+ ATPase alpha 1 antibody [SQab1875] WB image
Western blot: 10 µg of 293, HeLa, MCF7, Raji, TT and HCT-116 cell lysates stained with ARG66327 anti-ATP1A1 / Na+ K+ ATPase alpha 1 antibody [SQab1875] at 1:2000 dilution.
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ARG66327 anti-ATP1A1 / Na+ K+ ATPase alpha 1 antibody [SQab1875] IP image
Immunoprecipitation: 0.4 mg of TT whole cell lysate immunoprecipitated (1:50) and stained with ARG66327 anti-ATP1A1 / Na+ K+ ATPase alpha 1 antibody [SQab1875]. 1) ARG66327 IP in TT whole cell lysate, 2) PBS instead of ARG66327 in TT whole cell lysate, and 3) TT whole cell lysate, 10 µg (input).
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ARG66327 anti-ATP1A1 / Na+ K+ ATPase alpha 1 antibody [SQab1875] WB image
Western blot: 7 µg of PC-12 and Raw264.7 cell lysates stained with ARG66327 anti-ATP1A1 / Na+ K+ ATPase alpha 1 antibody [SQab1875] at 1:2000 dilution.
