ARG41952
anti-ATP1A / Na+ K+ ATPase alpha antibody
anti-ATP1A / Na+ K+ ATPase alpha antibody for IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse
Overview
| Product Description | Rabbit Polyclonal antibody recognizes ATP1A / Na+ K+ ATPase alpha |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | IHC-P, IP, WB |
| Specificity | This antibody is predicted to react with ATP1A1, ATP1A2, ATP1A3 and ATP1A4. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | ATP1A / Na+ K+ ATPase alpha |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 551-850 of Human ATP1A1 (NP_000692.2). |
| Conjugation | Un-conjugated |
| Alternate Names | Sodium pump subunit alpha; Sodium/potassium-transporting ATPase subunit alpha |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | HeLa | ||||||||
| Observed Size | ~ 113kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P05023 Human Sodium/potassium-transporting ATPase subunit alpha-1 Swiss-port # Q8VDN2 Mouse Sodium/potassium-transporting ATPase subunit alpha-1 |
|---|---|
| Gene Symbol | ATP1A |
| Gene Full Name | ATPase, Na+/K+ transporting, alpha |
| Background | ATP1A protein belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009] |
| Function | ATP1A is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients. [UniProt] |
| Cellular Localization | Cell membrane, Multi-pass membrane protein, Melanosome. [UniProt] |
| Calculated MW | 113 kDa |
| PTM | Phosphorylation on Tyr-10 modulates pumping activity. Phosphorylation of Ser-943 by PKA modulates the response of ATP1A1 to PKC. Dephosphorylation by protein phosphatase 2A (PP2A) following increases in intracellular sodium, leading to increase catalytic activity (By similarity). [UniProt] |
Images (3) Click the Picture to Zoom In
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ARG41952 anti-ATP1A / Na+ K+ ATPase alpha antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human prostate tissue stained with ARG41952 anti-ATP1A / Na+ K+ ATPase alpha antibody at 1:100 dilution.
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ARG41952 anti-ATP1A / Na+ K+ ATPase alpha antibody WB image
Western blot: 25 µg of HeLa whole cell lysate stained with ARG41952 anti-ATP1A / Na+ K+ ATPase alpha antibody at 1:500 dilution.
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ARG41952 anti-ATP1A / Na+ K+ ATPase alpha antibody IP image
Immunoprecipitation: 200 µg extracts of LO2 cells were immunoprecipitated and stained with ARG41952 anti-ATP1A / Na+ K+ ATPase alpha antibody at 1:1000 dilution.
