ARG66448

anti-ATG9B antibody

anti-ATG9B antibody for Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes ATG9B
Tested Reactivity Hu, Ms, Rat
Tested Application WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name ATG9B
Antigen Species Human
Immunogen Recombinant full length protein of Human ATG9B.
Conjugation Un-conjugated
Alternate Names APG9-like 2; SONE; NOS3AS; Autophagy-related protein 9B; Protein sONE; APG9L2; Nitric oxide synthase 3-overlapping antisense gene protein

Application Instructions

Application Suggestion
Tested Application Dilution
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size ~ 100 kDa

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer 0.42% Potassium phosphate (pH 7.3), 0.87% NaCl, 0.01% Sodium azide and 30% Glycerol.
Preservative 0.01% Sodium azide
Stabilizer 30% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 213948 Mouse ATG9B

GeneID: 285973 Human ATG9B

Swiss-port # Q674R7 Human Autophagy-related protein 9B

Swiss-port # Q6EBV9 Mouse Autophagy-related protein 9B

Gene Symbol ATG9B
Gene Full Name autophagy related 9B
Background This gene functions in the regulation of autophagy, a lysosomal degradation pathway. This gene also functions as an antisense transcript in the posttranscriptional regulation of the endothelial nitric oxide synthase 3 gene, which has 3' overlap with this gene on the opposite strand. Mutations in this gene and disruption of the autophagy process have been associated with multiple cancers. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2012]
Function Involved in autophagy and cytoplasm to vacuole transport (Cvt) vesicle formation. Plays a key role in the organization of the preautophagosomal structure/phagophore assembly site (PAS), the nucleating site for formation of the sequestering vesicle (By similarity). [UniProt]
Cellular Localization Cytoplasmic vesicle, autophagosome membrane; Multi-pass membrane protein. Note=Under amino acid starvation or rapamycin treatment, redistributes from a juxtanuclear clustered pool to a dispersed peripheral cytosolic pool. The starvation-induced redistribution depends on ULK1 and ATG13. [UniProt]
Calculated MW 101 kDa

Images (1) Click the Picture to Zoom In

  • ARG66448 anti-ATG9B antibody WB image

    Western blot: HepG2, HL60, Mouse liver and Rat brain lysates stained with ARG66448 anti-ATG9B antibody.