ARG59749
anti-ATG9B antibody
anti-ATG9B antibody for ICC/IF,Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes ATG9B |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat |
| Tested Application | ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | ATG9B |
| Antigen Species | Human |
| Immunogen | A 17 amino acid peptide within aa. 850 - 900 of Human ATG9B. |
| Conjugation | Un-conjugated |
| Alternate Names | APG9-like 2; SONE; NOS3AS; Autophagy-related protein 9B; Protein sONE; APG9L2; Nitric oxide synthase 3-overlapping antisense gene protein |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | HeLa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS and 0.02% Sodium azide |
| Preservative | 0.02% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ATG9B |
| Gene Full Name | autophagy related 9B |
| Background | This gene functions in the regulation of autophagy, a lysosomal degradation pathway. This gene also functions as an antisense transcript in the posttranscriptional regulation of the endothelial nitric oxide synthase 3 gene, which has 3' overlap with this gene on the opposite strand. Mutations in this gene and disruption of the autophagy process have been associated with multiple cancers. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2012] |
| Function | Involved in autophagy and cytoplasm to vacuole transport (Cvt) vesicle formation. Plays a key role in the organization of the preautophagosomal structure/phagophore assembly site (PAS), the nucleating site for formation of the sequestering vesicle (By similarity). [UniProt] |
| Cellular Localization | Cytoplasmic vesicle, autophagosome membrane; Multi-pass membrane protein. Note=Under amino acid starvation or rapamycin treatment, redistributes from a juxtanuclear clustered pool to a dispersed peripheral cytosolic pool. The starvation-induced redistribution depends on ULK1 and ATG13. [UniProt] |
| Calculated MW | 101 kDa |
Images (3) Click the Picture to Zoom In
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ARG59749 anti-ATG9B antibody ICC/IF image
Immunofluorescence: HeLa cells stained with ARG59749 anti-ATG9B antibody at 20 µg/ml dilution.
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ARG59749 anti-ATG9B antibody WB image
Western blot: HeLa cell lysate stained with ARG59749 anti-ATG9B antibody at 1 and 2 µg/ml dilution.
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ARG59749 anti-ATG9B antibody ICC image
Immunocytochemistry: HeLa cells stained with ARG59749 anti-ATG9B antibody at 10 µg/ml dilution.
