ARG57291
anti-ATG13 antibody
anti-ATG13 antibody for ICC/IF,Western blot and Human,Mouse,Rat
1
Overview
| Product Description | Rabbit Polyclonal antibody recognizes ATG13 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | ATG13 |
| Antigen Species | Human |
| Immunogen | Recombinant Protein of Human ATG13. |
| Conjugation | Un-conjugated |
| Alternate Names | KIAA0652; Autophagy-related protein 13; PARATARG8 |
Application Instructions
| Application Suggestion |
|
||||||
|---|---|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ATG13 |
| Gene Full Name | autophagy related 13 |
| Function | Autophagy factor required for autophagosome formation and mitophagy. Target of the TOR kinase signaling pathway that regulates autophagy through the control of the phosphorylation status of ATG13 and ULK1, and the regulation of the ATG13-ULK1-RB1CC1 complex. Through its regulation of ULK1 activity, plays a role in the regulation of the kinase activity of mTORC1 and cell proliferation. [UniProt] |
| Calculated MW | 57 kDa |
| PTM | Phosphorylated by ULK1, ULK2 and mTOR. Phosphorylation status depends on nutrient-rich conditions; dephosphorylated during starvation or following treatment with rapamycin. ULK1-mediated phosphorylation of ATG13 at Ser-355 is required for efficient clearance of depolarized mitochondria. |
Images (2) Click the Picture to Zoom In
-
ARG57291 anti-ATG13 antibody WB image
Western blot: 20 µg of HeLa and 3T3 cell lysates stained with ARG57291 anti-ATG13 antibody at 1:1000 dilution.
-
ARG57291 anti-ATG13 antibody ICC/IF image
Immunofluorescence: A549 cells stained with ARG57291 anti-ATG13 antibody.
Customer's Feedback
Excellent
Review for anti-ATG13 antibody
Application：WB
Sample：HeLa and 3T3
Sample Loading Amount：20 µg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
