ARG55295
anti-ATF4 antibody
anti-ATF4 antibody for Western blot and Human
Gene Regulation antibody
1
Overview
| Product Description | Rabbit Polyclonal antibody recognizes ATF4 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | ATF4 |
| Antigen Species | Human |
| Immunogen | Recombinant protein of Human ATF4 |
| Conjugation | Un-conjugated |
| Alternate Names | DNA-binding protein TAXREB67; TAXREB67; Tax-responsive enhancer element-binding protein 67; cAMP-dependent transcription factor ATF-4; Cyclic AMP-dependent transcription factor ATF-4; Activating transcription factor 4; CREB2; CREB-2; Cyclic AMP-responsive element-binding protein 2; cAMP-responsive element-binding protein 2; TXREB; TaxREB67 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P18848 Human Cyclic AMP-dependent transcription factor ATF-4 |
|---|---|
| Gene Symbol | ATF4 |
| Gene Full Name | activating transcription factor 4 |
| Background | This gene encodes a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromosome at q28 in a region containing a large inverted duplication. [provided by RefSeq, Sep 2011] |
| Function | Transcriptional activator. Binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Cooperates with FOXO1 in osteoblasts to regulate glucose homeostasis through suppression of beta-cell production and decrease in insulin production (By similarity). It binds to a Tax-responsive enhancer element in the long terminal repeat of HTLV-I. Regulates the induction of DDIT3/CHOP and asparagine synthetase (ASNS) in response to ER stress. In concert with DDIT3/CHOP, activates the transcription of TRIB3 and promotes ER stress-induced neuronal apoptosis by regulating the transcriptional induction of BBC3/PUMA. Activates transcription of SIRT4. Regulates the circadian expression of the core clock component PER2 and the serotonin transporter SLC6A4. Binds in a circadian time-dependent manner to the cAMP response elements (CRE) in the SLC6A4 and PER2 promoters and periodically activates the transcription of these genes. [UniProt] |
| Highlight | Related products: ATF4 antibodies; Anti-Rabbit IgG secondary antibodies; |
| Research Area | Gene Regulation antibody |
| Calculated MW | 39 kDa |
| PTM | Ubiquitinated by SCF(BTRC) in response to mTORC1 signal, followed by proteasomal degradation and leading to down-regulate expression of SIRT4. Phosphorylated by NEK6 (By similarity). Phosphorylated on the betaTrCP degron motif at Ser-219, followed by phosphorylation at Thr-213, Ser-224, Ser-231, Ser-235 and Ser-248, promoting interaction with BTRC and ubiquitination. Phosphorylation is promoted by mTORC1 (By similarity). Phosphorylated by NEK6. |
Images (1) Click the Picture to Zoom In
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ARG55295 anti-ATF4 antibody WB image
Western blot: 30 µg of PC3, Mouse brain and Rat brain lysates stained with ARG55295 anti-ATF4 antibody at 1:500 dilution.
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Review for anti-ATF4 antibody
Application：WB
Sample：
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
