ARG43903
anti-ATF4 antibody
anti-ATF4 antibody for ELISA,Flow cytometry,ICC/IF,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes ATF4 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ELISA, FACS, ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | ATF4 |
| Antigen Species | Human |
| Immunogen | Human ATF4 recombinant protein |
| Expression System | E.coli |
| Conjugation | Un-conjugated |
| Protein Full Name | Cyclic AMP-dependent transcription factor ATF-4 |
| Alternate Names | ATF4; Activating Transcription Factor 4; CREB-2; TAXREB67; TXREB; Tax-Responsive Enhancer Element-Binding Protein 67; Cyclic AMP-Dependent Transcription Factor ATF-4; Cyclic AMP-Responsive Element-Binding Protein 2; CAMP-Dependent Transcription Factor ATF-4; CAMP-Responsive Element-Binding Protein 2; Tax-Responsive Enhancer Element B67; CREB2; Activating Transcription Factor 4 (Tax-Responsive Enhancer Element B67); CAMP Response Element-Binding Protein 2; DNA-Binding Protein TAXREB67; TaxREB67 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Buffer | 0.9% NaCl, 0.2% Na2HPO4 and 4% Trehalose. |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ATF4 |
| Gene Full Name | Activating Transcription Factor 4 |
| Background | This gene encodes a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromosome at q28 in a region containing a large inverted duplication. [provided by RefSeq, Sep 2011] |
| Function | Transcription factor that binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3') and displays two biological functions, as regulator of metabolic and redox processes under normal cellular conditions, and as master transcription factor during integrated stress response (ISR). |
| Cellular Localization | Cell membrane, Cytoplasm, Cytoskeleton, Membrane, Nucleus |
| Calculated MW | 39 kDa |
| PTM | Acetylation, Hydroxylation, Isopeptide bond, Phosphoprotein, Ubl conjugation |
Images (5) Click the Picture to Zoom In
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ARG43903 anti-ATF4 antibody ICC/IF image
Immunofluorescence: Hela cells stained with ARG43903 anti-ATF4 antibody antibody at 5 μg/ml dilution.
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ARG43903 anti-ATF4 antibody WB image
Western blot: HepG2 stained with ARG43903 anti-ATF4 antibody at 0.5 μg/mL dilution.
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ARG43903 anti-ATF4 antibody FACS image
Flow Cytometry: SiHa cells stained with ARG43903 anti-ATF4 antibody (blue) at 1 μg/1x10^6 cells dilution.
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ARG43903 anti-ATF4 antibody WB image
Western blot: Rat brain stained with ARG43903 anti-ATF4 antibody at 0.5 μg/mL dilution.
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ARG43903 anti-ATF4 antibody WB image
Western blot: Mouse brain stained with ARG43903 anti-ATF4 antibody at 0.5 μg/mL dilution.
