ARG64203
anti-ASS1 / Argininosuccinate synthetase 1 antibody
anti-ASS1 / Argininosuccinate synthetase 1 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Metabolism antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes ASS1 / Argininosuccinate synthetase 1 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Predict Reactivity | Cow, Dog |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Specificity | The variants represent identical protein (NP_000041.2 and NP_446464.1). |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | ASS1 / Argininosuccinate synthetase 1 |
| Antigen Species | Human |
| Immunogen | C-ENPKNQAPPGLYTKTQD |
| Conjugation | Un-conjugated |
| Alternate Names | ASS; CTLN1; Citrulline--aspartate ligase; Argininosuccinate synthase; EC 6.3.4.5 |
Application Instructions
| Application Suggestion |
|
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ASS1 |
| Gene Full Name | argininosuccinate synthase 1 |
| Background | The protein encoded by this gene catalyzes the penultimate step of the arginine biosynthetic pathway. There are approximately 10 to 14 copies of this gene including the pseudogenes scattered across the human genome, among which the one located on chromosome 9 appears to be the only functional gene for argininosuccinate synthetase. Mutations in the chromosome 9 copy of this gene cause citrullinemia. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Aug 2012] |
| Function | One of the enzymes of the urea cycle, the metabolic pathway transforming neurotoxic amonia produced by protein catabolism into inocuous urea in the liver of ureotelic animals. Catalyzes the formation of arginosuccinate from aspartate, citrulline and ATP and together with ASL it is responsible for the biosynthesis of arginine in most body tissues. [UniProt] |
| Cellular Localization | Cytoplasm, cytosol. [UniProt] |
| Research Area | Metabolism antibody; Signaling Transduction antibody |
| Calculated MW | 47 kDa |
Images (7) Click the Picture to Zoom In
-
ARG64203 anti-ASS1 / Argininosuccinate synthetase 1 antibody ICC/IF image
Immunofluorescence: Paraformaldehyde-fixed HeLa cells permeabilized with 0.15% Triton. Cells were stained with ARG64203 anti-ASS1 / Argininosuccinate synthetase 1 antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
-
ARG64203 anti-ASS1 / Argininosuccinate synthetase 1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human kidney tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64203 anti-ASS1 / Argininosuccinate synthetase 1 antibody at 2.5 µg/ml dilution followed by AP-staining.
-
ARG64203 anti-ASS1 / Argininosuccinate synthetase 1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human liver tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64203 anti-ASS1 / Argininosuccinate synthetase 1 antibody at 2.5 µg/ml dilution followed by AP-staining.
-
ARG64203 anti-ASS1 / Argininosuccinate synthetase 1 antibody WB image
Western blot: 35 µg of A431 (A) and NIH/3T3 (B) cell lysates (in RIPA buffer) stained with ARG64203 anti-ASS1 / Argininosuccinate synthetase 1 antibody at 0.3 µg/ml (A) and 1 µg/ml (B) dilutions and incubated at RT for 1 hour.
-
ARG64203 anti-ASS1 / Argininosuccinate synthetase 1 antibody FACS image
Flow Cytometry: Paraformaldehyde-fixed A431 cells permeabilized with 0.5% Triton. Cells were stained with ARG64203 anti-ASS1 / Argininosuccinate synthetase 1 antibody (blue line) at 10 µg/ml dilution for 1 hour, followed by incubation with Alexa FluorR 488 labelled secondary antibody. IgG control: Unimmunized goat IgG (black line).
-
ARG64203 anti-ASS1 / Argininosuccinate synthetase 1 antibody WB image
Western blot: 35 µg of Human kidney (A), Mouse liver (B) and Rat kidney (C) lysates (in RIPA buffer) stained with ARG64203 anti-ASS1 / Argininosuccinate synthetase 1 antibody at 0.01 µg/ml (A, B) and 0.03 µg/ml (C) dilutions and incubated at RT for 1 hour.
-
ARG64203 anti-ASS1 / Argininosuccinate synthetase 1 antibody WB image
Western blot: 35 µg of Human kidney lysate (protein in RIPA buffer) stained with ARG64203 anti-ASS1 / Argininosuccinate synthetase 1 antibody at 0.03 µg/ml dilution.
