Overview

Product Description	Rabbit Polyclonal antibody recognizes ASIC3
Tested Reactivity	Hu
Tested Application	WB
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Target Name	ASIC3
Antigen Species	Human
Immunogen	Synthetic peptide around the internal region of Human ASIC3.
Conjugation	Un-conjugated
Alternate Names	ACCN3; Neuronal amiloride-sensitive cation channel 3; TNaC1; DRASIC; Testis sodium channel 1; hTNaC1; ASIC3; Amiloride-sensitive cation channel 3; SLNAC1; Acid-sensing ion channel 3; hASIC3

Application Instructions

Application Suggestion	Tested Application Dilution WB 1:500 - 1:2000
Application Note	* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size	~ 60 kDa

Properties

Form	Liquid
Purification	Affinity purification with immunogen.
Buffer	PBS, 0.02% Sodium azide, 50% Glycerol and 0.5% BSA.
Preservative	0.02% Sodium azide
Stabilizer	50% Glycerol and 0.5% BSA
Concentration	1 mg/ml
Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 9311 Human ASIC3 Swiss-port # Q9UHC3 Human Acid-sensing ion channel 3
Gene Symbol	ASIC3
Gene Full Name	acid sensing (proton gated) ion channel 3
Background	This gene encodes a member of the degenerin/epithelial sodium channel (DEG/ENaC) superfamily. The members of this family are amiloride-sensitive sodium channels that contain intracellular N and C termini, two hydrophobic transmembrane regions, and a large extracellular loop, which has many cysteine residues with conserved spacing. The member encoded by this gene is an acid sensor and may play an important role in the detection of lasting pH changes. In addition, a heteromeric association between this member and acid-sensing (proton-gated) ion channel 2 has been observed as proton-gated channels sensitive to gadolinium. Alternatively spliced transcript variants have been described. [provided by RefSeq, Feb 2012]
Function	Cation channel with high affinity for sodium, which is gated by extracellular protons and inhibited by the diuretic amiloride. Generates a biphasic current with a fast inactivating and a slow sustained phase. In sensory neurons is proposed to mediate the pain induced by acidosis that occurs in ischemic, damaged or inflamed tissue. May be involved in hyperalgesia. May play a role in mechanoreception. Heteromeric channel assembly seems to modulate channel properties. [UniProt]
Cellular Localization	Cell membrane; Multi-pass membrane protein. Cytoplasm. Note=Cell surface expression may be stabilized by interaction with LIN7B and cytoplasmic retention by interaction with DLG4. In part cytoplasmic in cochlea cells (By similarity). [UniProt]
Calculated MW	59 kDa
PTM	Phosphorylated by PKA. Phosphorylated by PKC. In vitro, PRKCABP/PICK-1 is necessary for PKC phosphorylation and activation of a ASIC3/ACCN3-ASIC2/ASIC2b channel, but does not activate a homomeric ASIC3 channel (By similarity). [UniProt]

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