ARG45558
anti-ASCL1 / MASH1 antibody
anti-ASCL1 / MASH1 antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes ASCL1 / MASH1 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | ASCL1 / MASH1 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide corresponding to C-terminal region of human ASCL1 / MASH1. |
| Conjugation | Un-conjugated |
| Alternate Names | ASCL1; Achaete-Scute Family BHLH Transcription Factor 1; HASH1; BHLHa46; ASH1; Class A Basic Helix-Loop-Helix Protein 46; Achaete-Scute Homolog 1; ASH-1; Achaete-Scute Complex (Drosophila) Homolog-Like 1; Achaete-Scute Complex Homolog 1 (Drosophila); Achaete-Scute Complex-Like 1 (Drosophila); Achaete-Scute Complex Homolog 1; Achaete-Scute Complex-Like 1; Achaete Scute Protein; BHLHA46; MASH1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Observed Size | 30 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ASCL1 |
| Gene Full Name | Achaete-Scute Family BHLH Transcription Factor 1 |
| Background | This gene encodes a member of the basic helix-loop-helix (BHLH) family of transcription factors. The protein activates transcription by binding to the E box (5'-CANNTG-3'). Dimerization with other BHLH proteins is required for efficient DNA binding. This protein plays a role in the neuronal commitment and differentiation and in the generation of olfactory and autonomic neurons. Mutations in this gene may contribute to the congenital central hypoventilation syndrome (CCHS) phenotype in rare cases. [provided by RefSeq, Jul 2008] |
| Function | Transcription factor that plays a key role in neuronal differentiation: acts as a pioneer transcription factor, accessing closed chromatin to allow other factors to bind and activate neural pathways. Directly binds the E box motif (5'-CANNTG-3') on promoters and promotes transcription of neuronal genes. The combination of three transcription factors, ASCL1, POU3F2/BRN2 and MYT1L, is sufficient to reprogram fibroblasts and other somatic cells into induced neuronal (iN) cells in vitro. Plays a role at early stages of development of specific neural lineages in most regions of the CNS, and of several lineages in the PNS. Essential for the generation of olfactory and autonomic neurons. Acts synergistically with FOXN4 to specify the identity of V2b neurons rather than V2a from bipotential p2 progenitors during spinal cord neurogenesis, probably through DLL4-NOTCH signaling activation. Involved in the regulation of neuroendocrine cell development in the glandular stomach. [UniProt] |
| Cellular Localization | Nucleus. [UniProt] |
| Calculated MW | 25 kDa |
| PTM | Acetylation. [UniProt] |
Images (5) Click the Picture to Zoom In
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ARG45558 anti-ASCL1 / MASH1 antibody IHC-P image
Immunohistochemistry: Human lung cancer stained with ARG45558 anti-ASCL1 / MASH1 antibody at 2 μg/ml dilution.
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ARG45558 anti-ASCL1 / MASH1 antibody WB image
Western blot: K562 stained with ARG45558 anti-ASCL1 / MASH1 antibody at 0.5 μg/ml dilution.
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ARG45558 anti-ASCL1 / MASH1 antibody WB image
Western blot: Rat brain stained with ARG45558 anti-ASCL1 / MASH1 antibody at 0.5 μg/ml dilution.
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ARG45558 anti-ASCL1 / MASH1 antibody FACS image
Flow Cytometry: K562 stained with ARG45558 anti-ASCL1 / MASH1 antibody at 1 µg/10^6 cells dilution.
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ARG45558 anti-ASCL1 / MASH1 antibody WB image
Western blot: Mouse brain stained with ARG45558 anti-ASCL1 / MASH1 antibody at 0.5 μg/ml dilution.
