ARG40258
anti-ASC1 antibody
anti-ASC1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes ASC1 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | IHC-P, IP, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | ASC1 |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 282-581 of Human ASC1 (NP_057297.2). |
| Conjugation | Un-conjugated |
| Alternate Names | TRIP-4; ZC2HC5; TR-interacting protein 4; HsT17391; ASC-1; ASC1; Activating signal cointegrator 1; Thyroid receptor-interacting protein 4 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | A549, Mouse brain and Rat brain | ||||||||
| Observed Size | 70 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | TRIP4 |
| Gene Full Name | thyroid hormone receptor interactor 4 |
| Function | Transcription coactivator which associates with nuclear receptors, transcriptional coactivators including EP300, CREBBP and NCOA1, and basal transcription factors like TBP and TFIIA to facilitate nuclear receptors-mediated transcription. May thereby play an important role in establishing distinct coactivator complexes under different cellular conditions. Plays a role in thyroid hormone receptor and estrogen receptor transactivation. Also involved in androgen receptor transactivation (By similarity). Plays a pivotal role in the transactivation of NF-kappa-B, SRF and AP1. Acts as a mediator of transrepression between nuclear receptor and either AP1 or NF-kappa-B. [UniProt] |
| Cellular Localization | Nucleus. Cytoplasm, cytosol. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Cytoplasmic under conditions of serum deprivation (PubMed:10454579). Colocalizes with NEK6 in the centrosome (PubMed:20873783). [UniProt] |
| Calculated MW | 66 kDa |
| PTM | Phosphorylated by NEK6. Polyufmylated by the UFM1-conjugating system composed of the enzymes UBA5, UFC1 and UFL1. Deufmylated by the protease UFSP2. Ufmylation of TRIP4 is promoted by ligand-bound nuclear receptors that compete with UFSP2 for interaction with TRIP4. Nuclear receptors-induced ufmylation promotes the recruitment of additional transcriptional coactivators like EP300 and NCOA1 and therefore the assembly of a coactivator complex facilitating nuclear receptor-mediated transcription. [UniProt] |
Images (4) Click the Picture to Zoom In
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ARG40258 anti-ASC1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human colon carcinoma tissue stained with ARG40258 anti-ASC1 antibody at 1:100 dilution.
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ARG40258 anti-ASC1 antibody WB image
Western blot: 25 µg of Mouse brain and A549 cell lysates stained with ARG40258 anti-ASC1 antibody at 1:1000 dilution.
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ARG40258 anti-ASC1 antibody IP image
Immunoprecipitation: 200 µg extracts of A549 cells were immunoprecipitated and stained with ARG40258 anti-ASC1 antibody at 1:1000 dilution.
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ARG40258 anti-ASC1 antibody WB image
Western blot: 25 µg of Rat brain lysate stained with ARG40258 anti-ASC1 antibody at 1:1000 dilution.
