ARG44004

anti-ASC / TMS1 antibody

anti-ASC / TMS1 antibody for ELISA,Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Overview

Product Description Rabbit Polyclonal antibody recognizes ASC / TMS1
Tested Reactivity Hu
Tested Application ELISA, FACS, ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name ASC / TMS1
Antigen Species Human
Immunogen Human ASC / TMS1 recombinant protein
Expression System E.coli
Conjugation Un-conjugated
Protein Full Name Apoptosis-associated speck-like protein containing a CARD
Alternate Names PYCARD; PYD And CARD Domain Containing; CARD5; ASC; Apoptosis-Associated Speck-Like Protein Containing A CARD; TMS-1; Caspase Recruitment Domain-Containing Protein 5; Target Of Methylation-Induced Silencing 1; TMS1; PYD And CARD Domain-Containing Protein; Apoptosis-Associated Speck-Like; HASC; TMS

Application Instructions

Application Suggestion
Tested Application Dilution
ELISA0.1-0.5 µg/ml
FACS1-3 µg/1x10^6
ICC/IF5 µg/ml
IHC-P2-5 µg/ml
WB0.1-0.25 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purified with Immunogen.
Buffer 0.9% NaCl, 0.2% Na2HPO4 and 4% Trehalose.
Stabilizer 4% Trehalose
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 29108 Human PYCARD

Swiss-port # Q9ULZ3 Human Apoptosis-associated speck-like protein containing a CARD

Gene Symbol PYCARD
Gene Full Name PYD And CARD Domain Containing
Background This gene encodes an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein is localized to the cytoplasm; however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. Two transcript variants encoding different isoforms have been found for this gene.
Function Functions as key mediator in apoptosis and inflammation.
Cellular Localization Cytoplasm, Endoplasmic reticulum, Golgi apparatus, Inflammasome, Membrane, Mitochondrion, Nucleus
Calculated MW 22 kDa
PTM Isopeptide bond, Phosphoprotein, Ubl conjugation

Images (4) Click the Picture to Zoom In

  • ARG44004 anti-ASC / TMS1 antibody IHC-P image

    Immunohistochemistry: Human urothelial carcinoma stained with ARG44004 anti-ASC / TMS1 antibody at 2 μg/ml dilution.

  • ARG44004 anti-ASC / TMS1 antibody WB image

    Western blot:THP-1 and HL-6 stained withARG44004 anti-ASC / TMS1 antibody at 0.5 μg/mL dilution.

  • ARG44004 anti-ASC / TMS1 antibody ICC/IF image

    Immunofluorescence: A549 cells stained with ARG44004 anti-ASC / TMS1 antibody at 5 μg/ml dilution.

  • ARG44004 anti-ASC / TMS1 antibody FACS image

    Flow Cytometry: THP-1 cells stained with ARG44004 anti-ASC / TMS1 antibody (blue) at 1 μg/1x10^6 cells dilution.