ARG10956
anti-ASAP1 antibody [7B12]
anti-ASAP1 antibody [7B12] for ELISA,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Rat
Publication1
Overview
| Product Description | Mouse Monoclonal antibody [7B12] recognizes ASAP1 |
|---|---|
| Tested Reactivity | Hu, Rat |
| Tested Application | ELISA, IHC-Fr, IHC-P, IP, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 7B12 |
| Isotype | IgG2a |
| Target Name | ASAP1 |
| Antigen Species | Human |
| Immunogen | Recombinant Protein of Human ASAP1 with N-terminal GST tag. |
| Conjugation | Un-conjugated |
| Alternate Names | PAP; Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1; 130 kDa phosphatidylinositol 4,5-bisphosphate-dependent ARF1 GTPase-activating protein; ARF GTPase-activating protein 1; CENTB4; DDEF1; PIP2-dependent ARF1 GAP; PAG2; ZG14P; Differentiation-enhancing factor 1; AMAP1; ADP-ribosylation factor-directed GTPase-activating protein 1; DEF-1; Development and differentiation-enhancing factor 1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||||
| Positive Control | Human: HT29 and MDA-MB-231. Rat: ASAP1 is strongly expressed in ASML and weakly expressed in 1AS pancreatic carcinoma cells. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified by affinity chromatography. |
| Buffer | PBS and 0.02% Sodium azide. |
| Preservative | 0.02% Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q1AAU6 Rat Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 Swiss-port # Q9ULH1 Human Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 |
|---|---|
| Gene Symbol | ASAP1 |
| Gene Full Name | ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 |
| Background | This gene encodes an ADP-ribosylation factor (ARF) GTPase-activating protein. The GTPase-activating activity is stimulated by phosphatidylinositol 4,5-biphosphate (PIP2), and is greater towards ARF1 and ARF5, and lesser for ARF6. This gene maybe involved in regulation of membrane trafficking and cytoskeleton remodeling. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011] |
| Function | Possesses phosphatidylinositol 4,5-bisphosphate-dependent GTPase-activating protein activity for ARF1 (ADP ribosylation factor 1) and ARF5 and a lesser activity towards ARF6. May coordinate membrane trafficking with cell growth or actin cytoskeleton remodeling by binding to both SRC and PIP2. May function as a signal transduction protein involved in the differentiation of fibroblasts into adipocytes and possibly other cell types (By similarity). Plays a role in ciliogenesis. [UniProt] |
| Calculated MW | 125 kDa |
| PTM | Phosphorylated on tyrosine residues by SRC. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG10956 anti-ASAP1 antibody [7B12] IHC-P image
Immunohistochemistry: High expression ASAP1 NSCLC tissue (left) and Low expression ASAP1 NSCLC tissue (right) stained with ARG10956 anti-ASAP1 antibody [7B12].
From Qixian Zheng et al. (2023), doi: org/10.21203/rs.3.rs-3068684/v1, Fig. 1 & 2.
Specific References
Probing the Expression of ASAP1 and FAK in nonsmall cell lung cancer and their clinicopathological significance
IHC-P / Human
