ARG64778

anti-ARPC4 antibody

anti-ARPC4 antibody for Western blot and Human

Signaling Transduction antibody

Overview

Product Description Goat Polyclonal antibody recognizes ARPC4
Tested Reactivity Hu
Predict Reactivity Ms
Tested Application WB
Specificity This antibody is expected to recognise isoform a ( NP_005709.1).
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name ARPC4
Antigen Species Human
Immunogen C-ERHNKPEVEVR
Conjugation Un-conjugated
Alternate Names Actin-related protein 2/3 complex subunit 4; ARC20; p20-ARC; Arp2/3 complex 20 kDa subunit; P20-ARC

Application Instructions

Application Suggestion
Tested Application Dilution
WB0.01 - 0.03 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 10093 Human ARPC4

Swiss-port # P59998 Human Actin-related protein 2/3 complex subunit 4

Background This gene encodes one of seven subunits of the human Arp2/3 protein complex. This complex controls actin polymerization in cells and has been conserved throughout eukaryotic evolution. This gene encodes the p20 subunit, which is necessary for actin nucleation and high-affinity binding to F-actin. Alternative splicing results in multiple transcript variants. Naturally occurring read-through transcription exists between this gene and the downstream tubulin tyrosine ligase-like family, member 3 (TTLL3), which results in the production of a fusion protein. [provided by RefSeq, Nov 2010]
Research Area Signaling Transduction antibody
Calculated MW 20 kDa

Images (1) Click the Picture to Zoom In

  • ARG64778 anti-ARPC4 antibody WB image

    Western Blot: Human Lymph Node lysate (35 µg protein in RIPA buffer) stained with ARG64778 anti-ARPC4 antibody at 0.03 µg/ml dilution.