ARG63465
anti-ARPC2 antibody
anti-ARPC2 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes ARPC2 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat, Cow, Dog |
| Tested Application | ICC/IF, IHC-P, WB |
| Specificity | The reported variants NP_005722.1 and NP_690601.1 represent identical protein. |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | ARPC2 |
| Antigen Species | Human |
| Immunogen | C-EMKTITGKTFSSR |
| Conjugation | Un-conjugated |
| Alternate Names | Arp2/3 complex 34 kDa subunit; PNAS-139; p34-ARC; Actin-related protein 2/3 complex subunit 2; ARC34; p34-Arc; PRO2446 |
Application Instructions
| Application Suggestion |
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # O15144 Human Actin-related protein 2/3 complex subunit 2 |
|---|---|
| Background | This gene encodes one of seven subunits of the human Arp2/3 protein complex. The Arp2/3 protein complex has been implicated in the control of actin polymerization in cells and has been conserved through evolution. The exact role of the protein encoded by this gene, the p34 subunit, has yet to be determined. Two alternatively spliced variants have been characterized to date. Additional alternatively spliced variants have been described but their full length nature has not been determined. [provided by RefSeq, Jul 2008] |
| Research Area | Signaling Transduction antibody |
| Calculated MW | 34 kDa |
Images (4) Click the Picture to Zoom In
-
ARG63465 anti-ARPC2 antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed A431 cells permeabilized with 0.15% Triton. Cells were stained with ARG63465 anti-ARPC2 antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
-
ARG63465 anti-ARPC2 antibody IHC-P image
Immunohistochemistry: paraffin embedded Human Spleen. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG63465 anti-ARPC2 antibody at 3.8 µg/ml dilution followed by AP-staining.
-
ARG63465 anti-ARPC2 antibody WB image
Western blot: 35 µg of Caco-2 (A), HeLa (B) and Jurkat (C) cell lysates (in RIPA buffer) stained with ARG63465 anti-ARPC2 antibody at 0.5 µg/ml (A) and 0.1 µg/ml (B, C) dilutions and incubated at RT for 1 hour.
-
ARG63465 anti-ARPC2 antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton. Cells were stained with ARG63465 anti-ARPC2 antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
