ARG42096
anti-ARPC1A antibody
anti-ARPC1A antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Goat Polyclonal antibody recognizes ARPC1A |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Predict Reactivity | Cow, Pig |
| Tested Application | IHC-P, WB |
| Specificity | This antibody is expected to recognize both reported isoforms (NP_006400.2; NP_001177925.1). No cross-reactiviry expected with ARPC1B. |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | ARPC1A |
| Antigen Species | Human |
| Immunogen | Synthetic peptide around the C-terminal region of Human ARPC1A. (C-DFKTLESSIQGLR) (NP_006400.2; NP_001177925.1) |
| Conjugation | Un-conjugated |
| Alternate Names | SOP2L; Actin-related protein 2/3 complex subunit 1A; SOP2Hs; HEL-68; Arc40; SOP2-like protein |
Application Instructions
| Application Suggestion |
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| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Observed Size | ~ 42 kDa (Human) |
Properties
| Form | Liquid |
|---|---|
| Purification | Ammonium sulphate precipitation followed by affinity purification with immunogen. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q92747 Human Actin-related protein 2/3 complex subunit 1A Swiss-port # Q9R0Q6 Mouse Actin-related protein 2/3 complex subunit 1A |
|---|---|
| Gene Symbol | ARPC1A |
| Gene Full Name | actin related protein 2/3 complex, subunit 1A, 41kDa |
| Background | This gene encodes one of seven subunits of the human Arp2/3 protein complex. This subunit is a member of the SOP2 family of proteins and is most similar to the protein encoded by gene ARPC1B. The similarity between these two proteins suggests that they both may function as p41 subunit of the human Arp2/3 complex that has been implicated in the control of actin polymerization in cells. It is possible that the p41 subunit is involved in assembling and maintaining the structure of the Arp2/3 complex. Multiple versions of the p41 subunit may adapt the functions of the complex to different cell types or developmental stages. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010] |
| Function | Probably functions as component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. [UniProt] |
| Cellular Localization | Cytoplasm, cytoskeleton. [UniProt] |
| Calculated MW | 42 kDa (Human) |
Images (3) Click the Picture to Zoom In
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ARG42096 anti-ARPC1A antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human heart tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG42096 anti-ARPC1A antibody at 5 µg/ml dilution.
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ARG42096 anti-ARPC1A antibody WB image
Western blot: 35 µg of HeLa cell lysate (in RIPA buffer) stained with ARG42096 anti-ARPC1A antibody at 0.3 µg/ml dilution and incubated at RT for 1 hour.
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ARG42096 anti-ARPC1A antibody WB image
Western blot: 35 µg of fetal mouse brain and adult rat brain lysates (in RIPA buffer) stained with ARG42096 anti-ARPC1A antibody at 0.1 µg/ml dilution and incubated at RT for 1 hour.
