ARG64841
anti-ARNTL / BMAL1 antibody
anti-ARNTL / BMAL1 antibody for Western blot and Human
Cell Biology and Cellular Response antibody; Gene Regulation antibody; Metabolism antibody; Neuroscience antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes ARNTL / BMAL1 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat, Cow, Dog, Pig |
| Tested Application | WB |
| Specificity | This antibody is expected to recognize both reported isoforms (NP_001025444.1; NP_001025443.1). Reported variants represent identical protein (NP_001025443.1; NP_001169.3). |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | ARNTL / BMAL1 |
| Antigen Species | Human |
| Immunogen | REKITTNCYKFKIKD |
| Conjugation | Un-conjugated |
| Alternate Names | BMAL1; Basic-helix-loop-helix-PAS protein MOP3; Brain and muscle ARNT-like 1; bHLH-PAS protein JAP3; PAS domain-containing protein 3; Aryl hydrocarbon receptor nuclear translocator-like protein 1; MOP3; JAP3; Class E basic helix-loop-helix protein 5; bHLHe5; Member of PAS protein 3; PASD3; BMAL1c; TIC |
Application Instructions
| Application Suggestion |
|
||||
|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # O00327 Human Aryl hydrocarbon receptor nuclear translocator-like protein 1 |
|---|---|
| Background | The protein encoded by this gene is a basic helix-loop-helix protein that forms a heterodimer with CLOCK. This complex binds an E-box upstream of the PER1 gene, activating this gene and possibly other circadian rhythym-associated genes. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Research Area | Cell Biology and Cellular Response antibody; Gene Regulation antibody; Metabolism antibody; Neuroscience antibody |
| Calculated MW | 69 kDa |
| PTM | Ubiquitinated, leading to its proteasomal degradation. O-glycosylated; contains O-GlcNAc. O-glycosylation by OGT prevents protein degradation by inhibiting ubiquitination. It also stabilizes the CLOCK-ARNTL/BMAL1 heterodimer thereby increasing CLOCK-ARNTL/BMAL1-mediated transcription of genes in the negative loop of the circadian clock such as PER1/2/3 and CRY1/2. Acetylated on Lys-538 upon dimerization with CLOCK. Acetylation facilitates CRY1-mediated repression. Deacetylated by SIRT1, which may result in decreased protein stability. Phosphorylated upon dimerization with CLOCK. Phosphorylation enhances the transcriptional activity, alters the subcellular localization and decreases the stability of the CLOCK-ARNTL/BMAL1 heterodimer by promoting its degradation. Phosphorylation shows circadian variations in the liver with a peak between CT10 to CT14. Phosphorylation at Ser-90 by CK2 is essential for its nuclear localization, its interaction with CLOCK and controls CLOCK nuclear entry (By similarity). Dephosphorylation at Ser-78 is important for dimerization with CLOCK and transcriptional activity (PubMed:23229515). Sumoylated on Lys-259 upon dimerization with CLOCK. Predominantly conjugated to poly-SUMO2/3 rather than SUMO1 and the level of these conjugates undergo rhythmic variation, peaking at CT9-CT12. Sumoylation localizes it exclusively to the PML body and promotes its ubiquitination in the PML body, ubiquitin-dependent proteasomal degradation and the transcriptional activity of the CLOCK-ARNTL/BMAL1 heterodimer. |
Images (1) Click the Picture to Zoom In
-
ARG64841 anti-ARNTL / BMAL1 antibody WB image
Western blot: Human Brain (Cerebellum) lysate (35 µg protein in RIPA buffer) stained with ARG64841 anti-ARNTL / BMAL1 antibody at 0.3 µg/ml dilution.
