ARG64382
anti-ARNO / cytohesin 2 antibody
anti-ARNO / cytohesin 2 antibody for Western blot and Human
Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes ARNO / cytohesin 2 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat, Dog |
| Tested Application | WB |
| Specificity | This antibody is expected to recognise both reported isoforms (NP_004219.2; NP_059431.1). |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | ARNO / cytohesin 2 |
| Antigen Species | Human |
| Immunogen | EDGVYEPPDLTP-C |
| Conjugation | Un-conjugated |
| Alternate Names | Sec7p-like; Protein ARNO; CTS18; Cytohesin-2; PH, SEC7 and coiled-coil domain-containing protein 2; ARF nucleotide-binding site opener; Sec7p-L; PSCD2; SEC7L; ARNO; ARF exchange factor; PSCD2L; CTS18.1 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Observed Size | ~ 47 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | The protein encoded by this gene is a member of the PSCD family. Members of this family have identical structural organization that consists of an N-terminal coiled-coil motif, a central Sec7 domain, and a C-terminal pleckstrin homology (PH) domain. The coiled-coil motif is involved in homodimerization, the Sec7 domain contains guanine-nucleotide exchange protein (GEP) activity, and the PH domain interacts with phospholipids and is responsible for association of PSCDs with membranes. Members of this family appear to mediate the regulation of protein sorting and membrane trafficking. The encoded protein exhibits GEP activity in vitro with ARF1, ARF3, and ARF6 and is 83% homologous to CYTH1. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008] |
| Research Area | Signaling Transduction antibody |
| Calculated MW | 47 kDa |
Images (1) Click the Picture to Zoom In
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ARG64382 anti-ARNO / cytohesin 2 antibody WB image
Western blot: 35 µg of Human brain (cerebellum) lysate (in RIPA buffer) stained with ARG64382 anti-ARNO / cytohesin 2 antibody at 0.5 µg/ml dilution and incubated at RT for 1 hour.
