ARG58200
anti-ARF4 antibody
anti-ARF4 antibody for Western blot and Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes ARF4 |
|---|---|
| Tested Reactivity | Rat |
| Tested Application | WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | ARF4 |
| Antigen Species | Rat |
| Immunogen | Synthetic peptide corresponding to unique amino acid sequence on ARF4 protein. |
| Conjugation | Un-conjugated |
| Alternate Names | ARF2; ADP-ribosylation factor 4 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| Observed Size | ~ 21 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | Tris, HCl/glycine buffer (pH 7.4-7.8), cryo-protective agents, Hepes, 0.02% Sodium azide, 30% Glycerol and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 30% Glycerol and 0.5% BSA |
| Concentration | 0.75 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ARF4 |
| Gene Full Name | ADP-ribosylation factor 4 |
| Background | This gene is a member of the human ARF gene family whose members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 5 ARF proteins and 11 ARF-like proteins and constitute one family of the RAS superfamily. The ARF proteins are categorized as class I, class II and class III; this gene is a class II member. The members of each class share a common gene organization. The ARF4 gene spans approximately 12kb and contains six exons and five introns. This gene is the most divergent member of the human ARFs. Conflicting map positions at 3p14 or 3p21 have been reported for this gene. [provided by RefSeq, Jul 2008] |
| Function | GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus. [UniProt] |
| Calculated MW | 21 kDa |
Images (1) Click the Picture to Zoom In
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ARG58200 anti-ARF4 antibody WB image
Western blot: Rat hippocampus, Rat lung, ARF4 protein (positive control), Rat spleen and Rat kidney lysates stained with ARG58200 anti-ARF4 antibody at 1:250 dilution.
