ARG58223

anti-AREG / Amphiregulin antibody

anti-AREG / Amphiregulin antibody for Western blot and Human,Mouse

Overview

Product Description Rabbit Polyclonal antibody recognizes AREG / Amphiregulin
Tested Reactivity Hu, Ms
Tested Application WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name AREG / Amphiregulin
Antigen Species Human
Immunogen Recombinant fusion protein corresponding to aa. 20-252 of Human AREG (NP_001648.1).
Conjugation Un-conjugated
Alternate Names Amphiregulin; AREGB; CRDGF; SDGF; AR; Colorectum cell-derived growth factor

Application Instructions

Application Suggestion
Tested Application Dilution
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control MCF7
Observed Size 35 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 11839 Mouse AREG

GeneID: 374 Human AREG

Swiss-port # P15514 Human Amphiregulin

Swiss-port # P31955 Mouse Amphiregulin

Gene Symbol AREG
Gene Full Name amphiregulin
Background The protein encoded by this gene is a member of the epidermal growth factor family. It is an autocrine growth factor as well as a mitogen for astrocytes, Schwann cells and fibroblasts. It is related to epidermal growth factor (EGF) and transforming growth factor alpha (TGF-alpha). The protein interacts with the EGF/TGF-alpha receptor to promote the growth of normal epithelial cells, and it inhibits the growth of certain aggressive carcinoma cell lines. It also functions in mammary gland, oocyte and bone tissue development. This gene is associated with a psoriasis-like skin phenotype, and is also associated with other pathological disorders, including various types of cancers and inflammatory conditions. [provided by RefSeq, Apr 2014]
Function Ligand of the EGF receptor/EGFR. Autocrine growth factor as well as a mitogen for a broad range of target cells including astrocytes, Schwann cells and fibroblasts. [UniProt]
Cellular Localization Membrane, Single-pass membrane protein. [UniProt]
Calculated MW 28 kDa

Images (1) Click the Picture to Zoom In

  • ARG58223 anti-AREG / Amphiregulin antibody WB image

    Western blot: 25 µg of MCF7 cell lysate stained with ARG58223 anti-AREG / Amphiregulin antibody at 1:1000 dilution.