ARG43287
anti-AREG / Amphiregulin antibody
anti-AREG / Amphiregulin antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes AREG / Amphiregulin |
|---|---|
| Tested Reactivity | Ms, Rat |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | AREG / Amphiregulin |
| Antigen Species | Mouse |
| Immunogen | Recombinant protein corresponding to V100-K191 of Mouse AREG / Amphiregulin. |
| Conjugation | Un-conjugated |
| Alternate Names | Amphiregulin; AREGB; CRDGF; SDGF; AR; Colorectum cell-derived growth factor |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | Rat brain and Mouse brain | ||||||
| Observed Size | ~ 40 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 4% Trehalose. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | AREG |
| Gene Full Name | amphiregulin |
| Background | The protein encoded by this gene is a member of the epidermal growth factor family. It is an autocrine growth factor as well as a mitogen for astrocytes, Schwann cells and fibroblasts. It is related to epidermal growth factor (EGF) and transforming growth factor alpha (TGF-alpha). The protein interacts with the EGF/TGF-alpha receptor to promote the growth of normal epithelial cells, and it inhibits the growth of certain aggressive carcinoma cell lines. It also functions in mammary gland, oocyte and bone tissue development. This gene is associated with a psoriasis-like skin phenotype, and is also associated with other pathological disorders, including various types of cancers and inflammatory conditions. [provided by RefSeq, Apr 2014] |
| Function | Ligand of the EGF receptor/EGFR. Autocrine growth factor as well as a mitogen for a broad range of target cells including astrocytes, Schwann cells and fibroblasts. [UniProt] |
| Cellular Localization | Membrane; Single-pass membrane protein. [UniProt] |
| Calculated MW | 28 kDa |
Images (2) Click the Picture to Zoom In
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ARG43287 anti-AREG / Amphiregulin antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse kidney tissue. Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43287 anti-AREG / Amphiregulin antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG43287 anti-AREG / Amphiregulin antibody WB image
Western blot: 50 µg of sample under reducing conditions. Rat brain and Mouse brain lysates stained with ARG43287 anti-AREG / Amphiregulin antibody at 0.5 µg/ml dilution, overnight at 4°C.
