ARG63376

anti-ARAP3 antibody

anti-ARAP3 antibody for Immunoprecipitation,Western blot and Human

Signaling Transduction antibody

Overview

Product Description Goat Polyclonal antibody recognizes ARAP3
Tested Reactivity Hu
Predict Reactivity Ms, Cow, Dog, Pig
Tested Application IP, WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name ARAP3
Antigen Species Human
Immunogen CTSSPPSSQPLT
Conjugation Un-conjugated
Alternate Names Arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 3; CENTD3; Cnt-d3; DRAG1; Centaurin-delta-3

Application Instructions

Application Suggestion
Tested Application Dilution
IPAssay - dependent
WBAssay - dependent
Application Note WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 64411 Human ARAP3

Swiss-port # Q8WWN8 Human Arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 3

Background This gene encodes a phosphoinositide binding protein containing ARF-GAP, RHO-GAP, RAS-associating, and pleckstrin homology domains. The ARF-GAP and RHO-GAP domains cooperate in mediating rearrangements in the cell cytoskeleton and cell shape. It is a specific PtdIns(3,4,5)P3/PtdIns(3,4)P2-stimulated Arf6-GAP protein. An alternatively spliced transcript has been found for this gene, but its biological validity has not been determined. [provided by RefSeq, Sep 2008]
Research Area Signaling Transduction antibody
Calculated MW 170 kDa
PTM Tyrosine phosphorylated at a low basal level. PDGF treatment stimulates phosphorylation. Tyrosine phosphorylation is increased in cells that are in the process of becoming attached to a substrate and that start spreading and flattening (By similarity).