ARG62673
anti-ARAP1 antibody [ARAP1-2]
anti-ARAP1 antibody [ARAP1-2] for ICC/IF,Immunoprecipitation,Western blot and Human
Signaling Transduction antibody
Overview
| Product Description | Mouse Monoclonal antibody [ARAP1-2] recognizes ARAP1 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ICC/IF, IP, WB |
| Specificity | The clone ARAP1-2 reacts with C-terminal part of ARAP1, a 160 kDa adaptor protein. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | ARAP1-2 |
| Isotype | IgG1 |
| Target Name | ARAP1 |
| Antigen Species | Human |
| Immunogen | His6-tagged protein corresponding to amino acids 1190-1450 of human ARAP1 |
| Conjugation | Un-conjugated |
| Alternate Names | Arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 1; CENTD2; Cnt-d2; Centaurin-delta-2 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from ascites by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q96P48 Human Arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 1 |
|---|---|
| Gene Symbol | ARAP1 |
| Gene Full Name | ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1 |
| Background | ARAP1 (angiotensin II type 1 receptor-associated protein) is an adaptor protein with ArfGAP and RhoGAP activities, containing five PH domains and ankyrin repeate. This adaptor seems to serve as a link between phosphoinositide- Arf-, and Rho-mediated cell signaling. ARAP1 supports the plasma membrane recycling of angiotensin II type 1 receptor (AT1) and is important also for cell-specific trafficking of pro-death receptor TRAIL-R1 (DR4) to the plasma membrane, thus promoting the TRAIL-induced apoptosis in certain cell types. ARAP1 also affects cell spreading. |
| Function | Phosphatidylinositol 3,4,5-trisphosphate-dependent GTPase-activating protein that modulates actin cytoskeleton remodeling by regulating ARF and RHO family members. Is activated by phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3) binding. Can be activated by phosphatidylinositol 3,4-bisphosphate (PtdIns(3,4,5)P2) binding, albeit with lower efficiency. Has a preference for ARF1 and ARF5 (By similarity). [UniProt] |
| Research Area | Signaling Transduction antibody |
| Calculated MW | 162 kDa |
Images (1) Click the Picture to Zoom In
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ARG62673 anti-ARAP1 antibody [ARAP1-2] ICC/IF image
Immunofluorescence: ARAP1-transfected NCTC cells treated with TRAIL for 0´, 30´ or 60´ stained with ARG62673 anti-ARAP1 antibody [ARAP1-2] (green)
DR4 was detected by rabbit polyclonal antibody (red).
