ARG45581
anti-APPL1 antibody
anti-APPL1 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes APPL1 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | APPL1 |
| Antigen Species | Human |
| Immunogen | Recombinant protein containing to human APPL1. |
| Conjugation | Un-conjugated |
| Alternate Names | APPL1; Adaptor Protein, Phosphotyrosine Interacting With PH Domain And Leucine Zipper 1; APPL; DCC-Interacting Protein 13-Alpha; Adaptor Protein, Phosphotyrosine Interaction, PH Domain And Leucine Zipper Containing 1; Adapter Protein Containing PH Domain, PTB Domain And Leucine Zipper Motif 1; Dip13-Alpha; Adaptor Protein Containing PH Domain, PTB Domain And Leucine Zipper Motif 1; Signaling Adaptor Protein DIP13alpha; AKT2 Interactor; DIP13alpha; KIAA1428; MODY14; DIP13A |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| Observed Size | 85 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified |
| Buffer | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 4% Trehalose. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | APPL1 |
| Gene Full Name | Adaptor Protein, Phosphotyrosine Interacting With PH Domain And Leucine Zipper 1 |
| Background | The protein encoded by this gene has been shown to be involved in the regulation of cell proliferation, and in the crosstalk between the adiponectin signalling and insulin signalling pathways. The encoded protein binds many other proteins, including RAB5A, DCC, AKT2, PIK3CA, adiponectin receptors, and proteins of the NuRD/MeCP1 complex. This protein is found associated with endosomal membranes, but can be released by EGF and translocated to the nucleus. [provided by RefSeq, Jul 2008] |
| Function | Multifunctional adapter protein that binds to various membrane receptors, nuclear factors and signaling proteins to regulate many processes, such as cell proliferation, immune response, endosomal trafficking and cell metabolism. [UniProt] |
| Cellular Localization | Cell projection; Cytoplasm; Cytoplasmic vesicle; Endosome; Membrane; Nucleus. [UniProt] |
| Calculated MW | 80 kDa |
| PTM | Phosphoprotein. [UniProt] |
Images (6) Click the Picture to Zoom In
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ARG45581 anti-APPL1 antibody IHC-P image
Immunohistochemistry: Human tonsil stained with ARG45581 anti-APPL1 antibody at 1 μg/ml dilution.
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ARG45581 anti-APPL1 antibody ICC/IF image
Immunofluorescence: U20S stained with ARG45581 anti-APPL1 antibody at 5 μg/ml dilution.
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ARG45581 anti-APPL1 antibody WB image
Western blot: Hela stained with ARG45581 anti-APPL1 antibody at 0.5 μg/ml dilution.
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ARG45581 anti-APPL1 antibody FACS image
Flow Cytometry: A549 stained with ARG45581 anti-APPL1 antibody at 1 µg/10^6 cells dilution.
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ARG45581 anti-APPL1 antibody WB image
Western blot: Rat brain stained with ARG45581 anti-APPL1 antibody at 0.5 μg/ml dilution.
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ARG45581 anti-APPL1 antibody WB image
Western blot: Mouse brain stained with ARG45581 anti-APPL1 antibody at 0.5 μg/ml dilution.
