ARG64714

anti-APOM antibody

anti-APOM antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody

Overview

Product Description Goat Polyclonal antibody recognizes APOM
Tested Reactivity Hu
Predict Reactivity Ms, Rat
Tested Application IHC-P, WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name APOM
Antigen Species Mouse
Immunogen C-KGNMELRTEGRPD
Conjugation Un-conjugated
Alternate Names ApoM; Apo-M; NG20; Apolipoprotein M; G3a; Protein G3a; HSPC336; apo-M

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P3 - 5 µg/ml
WB0.3 - 1 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 55937 Human APOM

Swiss-port # O95445 Human Apolipoprotein M

Gene Symbol Apom
Gene Full Name apolipoprotein M
Background The protein encoded by this gene is an apolipoprotein and member of the lipocalin protein family. It is found associated with high density lipoproteins and to a lesser extent with low density lipoproteins and triglyceride-rich lipoproteins. The encoded protein is secreted through the plasma membrane but remains membrane-bound, where it is involved in lipid transport. Alternate splicing results in both coding and non-coding variants of this gene. [provided by RefSeq, Jan 2012]
Function Probably involved in lipid transport. Can bind sphingosine-1-phosphate, myristic acid, palmitic acid and stearic acid, retinol, all-trans-retinoic acid and 9-cis-retinoic acid (By similarity). [UniProt]
Research Area Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody
Calculated MW 21 kDa

Images (2) Click the Picture to Zoom In

  • ARG64714 anti-APOM antibody WB image

    Western blot: Human Placenta lysate (35 µg protein in RIPA buffer) stained with ARG64714 anti-APOM antibody at 0.5 µg/ml dilution.

  • ARG64714 anti-APOM antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human liver tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64714 anti-APOM antibody at 3.75 µg/ml dilution followed by AP-staining.