ARG64604
anti-APOL4 antibody
anti-APOL4 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cell Biology and Cellular Response antibody; Metabolism antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes APOL4 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-P, WB |
| Specificity | This antibody is expected to recognize both reported isoforms (NP_085146.2 and NP_663693.1). |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | APOL4 |
| Antigen Species | Human |
| Immunogen | C-ENLNELTHIHQSLK |
| Conjugation | Un-conjugated |
| Alternate Names | Apolipoprotein L-IV; APOLIV; Apolipoprotein L4; APOL-IV; ApoL-IV |
Application Instructions
| Application Suggestion |
|
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|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | The protein encoded by this gene is a member of the apolipoprotein L family and may play a role in lipid exchange and transport throughout the body, as well as in reverse cholesterol transport from peripheral cells to the liver. Two transcript variants encoding two different isoforms have been found for this gene. Only one of the isoforms appears to be a secreted protein. [provided by RefSeq, Jul 2008] |
| Research Area | Cell Biology and Cellular Response antibody; Metabolism antibody |
| Calculated MW | 39 kDa |
Images (2) Click the Picture to Zoom In
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ARG64604 anti-APOL4 antibody WB image
Western blot: Human Placenta lysate (35 µg protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. stained with ARG64604 anti-APOL4 antibody at 0.5 µg/ml dilution.
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ARG64604 anti-APOL4 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human skeletal muscle tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64604 anti-APOL4 antibody at 5 µg/ml dilution followed by AP-staining.
