ARG64766

anti-APOL1 antibody

anti-APOL1 antibody for Western blot and Human

Cell Biology and Cellular Response antibody; Metabolism antibody

Overview

Product Description Goat Polyclonal antibody recognizes APOL1
Tested Reactivity Hu
Tested Application WB
Specificity This antibody is expected to recognize both reported isoforms (NP_003652.2; NP_663318.1).
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name APOL1
Antigen Species Human
Immunogen C-NNNYKILQADQE
Conjugation Un-conjugated
Alternate Names APO-L; ApoL-I; APOL; FSGS4; Apolipoprotein L; Apolipoprotein L-I; Apolipoprotein L1; Apo-L; APOL-I; ApoL

Application Instructions

Application Suggestion
Tested Application Dilution
WB0.1 - 0.3 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 8542 Human APOL1

Swiss-port # O14791 Human Apolipoprotein L1

Background This gene encodes a secreted high density lipoprotein which binds to apolipoprotein A-I. Apolipoprotein A-I is a relatively abundant plasma protein and is the major apoprotein of HDL. It is involved in the formation of most cholesteryl esters in plasma and also promotes efflux of cholesterol from cells. This apolipoprotein L family member may play a role in lipid exchange and transport throughout the body, as well as in reverse cholesterol transport from peripheral cells to the liver. Several different transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2008]
Research Area Cell Biology and Cellular Response antibody; Metabolism antibody
Calculated MW 44 kDa
PTM Phosphorylated by FAM20C in the extracellular medium.

Images (1) Click the Picture to Zoom In

  • ARG64766 anti-APOL1 antibody WB image

    Western Blot: Human Placenta lysate (35 µg protein in RIPA buffer) stained with ARG64766 anti-APOL1 antibody at 0.1 µg/ml dilution.