ARG42925
anti-API5 / FIF antibody
anti-API5 / FIF antibody for Immunocytochemistry,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes API5 / FIF |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC, IHC-Fr, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | API5 / FIF |
| Antigen Species | Human |
| Immunogen | Synthetic peptide corresponding to aa. 487-504 of Human API5 / FIF. (KYSSNLGNFNYERSLQGK) |
| Conjugation | Un-conjugated |
| Alternate Names | Apoptosis inhibitor 5; Antiapoptosis clone 11 protein; API-5; AAC11; Protein XAGL; AAC-11; FIF; Fibroblast growth factor 2-interacting factor; Cell migration-inducing gene 8 protein |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Observed Size | ~ 55 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Thimerosal, 0.05% Sodium azide and 5% BSA. |
| Preservative | 0.05% Thimerosal and 0.05% Sodium azide |
| Stabilizer | 5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | API5 |
| Gene Full Name | apoptosis inhibitor 5 |
| Background | This gene encodes an apoptosis inhibitory protein whose expression prevents apoptosis after growth factor deprivation. This protein suppresses the transcription factor E2F1-induced apoptosis and also interacts with, and negatively regulates Acinus, a nuclear factor involved in apoptotic DNA fragmentation. Its depletion enhances the cytotoxic action of the chemotherapeutic drugs. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011] |
| Function | Antiapoptotic factor that may have a role in protein assembly. Negatively regulates ACIN1. By binding to ACIN1, it suppresses ACIN1 cleavage from CASP3 and ACIN1-mediated DNA fragmentation. Also known to efficiently suppress E2F1-induced apoptosis. Its depletion enhances the cytotoxic action of the chemotherapeutic drugs. [UniProt] |
| Cellular Localization | Nucleus. Cytoplasm. Note=Mainly nuclear. Can also be cytoplasmic. Isoform 3: Cytoplasm. [UniProt] |
| Calculated MW | 59 kDa |
| PTM | Acetylation at Lys-251 impairs antiapoptotic function. [UniProt] |
Images (7) Click the Picture to Zoom In
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ARG42925 anti-API5 / FIF antibody ICC image
Immunocytochemistry: HeLa cells stained with ARG42925 anti-API5 / FIF antibody.
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ARG42925 anti-API5 / FIF antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat brain tissue stained with ARG42925 anti-API5 / FIF antibody.
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ARG42925 anti-API5 / FIF antibody WB image
Western blot: Rat cardiac muscle, Rat brain, Rat testis, Rat placenta, MCF7, HeLa, CEM and SMMC-7721 cell lysates stained with ARG42925 anti-API5 / FIF antibody.
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ARG42925 anti-API5 / FIF antibody IHC-Fr image
Immunohistochemistry: Frozen section of Rat cardiac muscle tissue stained with ARG42925 anti-API5 / FIF antibody.
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ARG42925 anti-API5 / FIF antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human placenta tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG42925 anti-API5 / FIF antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG42925 anti-API5 / FIF antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat intestines tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG42925 anti-API5 / FIF antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG42925 anti-API5 / FIF antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse intestines tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG42925 anti-API5 / FIF antibody at 1 µg/ml dilution, overnight at 4°C.
