ARG55111
anti-APC5 antibody
anti-APC5 antibody for ELISA,ICC/IF,Immunohistochemistry,Western blot and Human,Mouse,Rat
Cell Biology and Cellular Response antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes APC5 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ELISA, ICC/IF, IHC, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | APC5 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide (17 aa) within aa. 480-530 of Human Apc5 protein. |
| Conjugation | Un-conjugated |
| Alternate Names | Anaphase-promoting complex subunit 5; Cyclosome subunit 5; APC5 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| Positive Control | Human Kidney Tissue Lysate |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS and 0.02% Sodium azide |
| Preservative | 0.02% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ANAPC5 |
| Gene Full Name | anaphase promoting complex subunit 5 |
| Background | APC5 Antibody: Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis. APC5 is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. The individual APC/C components mRNA and protein levels are expressed at approximately the same levels in most tissues and cell lines, suggesting that they perform their functions as part of a complex. While little is known of APC5, it is thought that APC5 associates with other APC/C components APC1, APC4, and CDC23 interdependently, such that loss of any one subunit reduces binding between the remaining three. |
| Function | Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. [UniProt] |
| Research Area | Cell Biology and Cellular Response antibody |
| Calculated MW | 85 kDa |
Images (3) Click the Picture to Zoom In
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ARG55111 anti-APC5 antibody WB image
Western blot: Human kidney tissue lysate stained with ARG55111 anti-APC5 antibody at (A) 1 and (B) 2 ug/ml dilution.
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ARG55111 anti-APC5 antibody IHC image
Immunohistochemistry: APC5 in Rat kidney tissue stained with ARG55111 anti-APC5 antibody at 20 ug/ml dilution.
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ARG55111 anti-APC5 antibody IHC image
Immunohistochemistry: APC5 in Rat kidney tissue stained with ARG55111 anti-APC5 antibody at 5 ug/ml dilution.
