ARG43635
anti-APC4 antibody
anti-APC4 antibody for Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes APC4 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | APC4 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide within a.a. 630-670 of Human APC4. |
| Conjugation | Un-conjugated |
| Protein Full Name | Anaphase-promoting complex subunit 4 |
| Alternate Names | Anaphase-promoting complex subunit 4; Cyclosome subunit 4; APC4 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| Positive Control | HepG2 | ||||
| Observed Size | 95-100 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A and immunogen peptide. |
| Buffer | PBS and 0.09% (W/V) Sodium azide. |
| Preservative | 0.09% (W/V) Sodium azide. |
| Concentration | Batch dependent |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9UJX5 Human Anaphase-promoting complex subunit 4 |
|---|---|
| Gene Symbol | ANAPC4 |
| Gene Full Name | anaphase promoting complex subunit 4 |
| Background | A large protein complex, termed the anaphase-promoting complex (APC), or the cyclosome, promotes metaphase-anaphase transition by ubiquitinating its specific substrates such as mitotic cyclins and anaphase inhibitor, which are subsequently degraded by the 26S proteasome. Biochemical studies have shown that the vertebrate APC contains eight subunits. The composition of the APC is highly conserved in organisms from yeast to humans. The exact function of this gene product is not known. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2013] |
| Function | Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. [UniProt] |
| Cellular Localization | Nucleus [UniProt] |
| Calculated MW | 92 kDa |
| PTM | Isopeptide bond; Phosphoprotein; Ubl conjugation |
Images (1) Click the Picture to Zoom In
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ARG43635 anti-APC4 antibody WB image
Western blot: HepG2 lysates were stained with ARG43635 anti-APC4 antibody at 1:1000 dilution.
