ARG43660
anti-APC11 antibody
anti-APC11 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Cell Biology and Cellular Response antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes APC11 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | APC11 |
| Antigen Species | Human |
| Immunogen | Recombinant fragment within a.a. 1-90 of Human APC11. |
| Conjugation | Un-conjugated |
| Protein Full Name | Anaphase-promoting complex subunit 11 |
| Alternate Names | Hepatocellular carcinoma-associated RING finger protein; APC11; Cyclosome subunit 11; Apc11p; HSPC214; Anaphase-promoting complex subunit 11 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | Mouse heart | ||||||||
| Observed Size | 15-20 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS, 0.01% Thiomersal and 50% Glycerol. |
| Preservative | 0.01% Thiomersal |
| Stabilizer | 50% Glycerol |
| Concentration | Batch dependent |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9CPX9 Mouse Anaphase-promoting complex subunit 11 Swiss-port # Q9NYG5 Human Anaphase-promoting complex subunit 11 |
|---|---|
| Gene Symbol | ANAPC11 |
| Gene Full Name | anaphase promoting complex subunit 11 |
| Background | Enables cullin family protein binding activity and ubiquitin-ubiquitin ligase activity. Contributes to ubiquitin-protein transferase activity. Involved in protein K11-linked ubiquitination. Located in nucleolus and nucleoplasm. Part of anaphase-promoting complex. [provided by Alliance of Genome Resources, Apr 2022] |
| Function | Together with the cullin protein ANAPC2, constitutes the catalytic component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. May recruit the E2 ubiquitin-conjugating enzymes to the complex. [UniProt] |
| Cellular Localization | Cytoplasm; Nucleus [UniProt] |
| Research Area | Cell Biology and Cellular Response antibody |
| Calculated MW | 10 kDa |
| PTM | Auto-ubiquitinated. |
Images (4) Click the Picture to Zoom In
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ARG43660 anti-APC11 antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human Liver tissue stained with ARG43660 anti-APC11 antibody at 1:150 dilution. Antigen Retrieval: Heat mediated was performed using 10 mM citrate buffer pH 6.0
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ARG43660 anti-APC11 antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Mouse Kidney tissue stained with ARG43660 anti-APC11 antibody at 1:150 dilution. Antigen Retrieval: Heat mediated was performed using 10 mM citrate buffer pH 6.0
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ARG43660 anti-APC11 antibody WB image
Western blot: Mouse Heart tissue stained with ARG43660 anti-APC11 antibody at 1:1000 dilution.
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ARG43660 anti-APC11 antibody ICC/IF image
Immunofluorescence: A549 cells stained with ARG43660 anti-APC11 antibody at 1:300 and 4°C. DAPI (blue) was used as the nuclear counter stain.
