ARG64035
anti-APBB1 / FE65 antibody
anti-APBB1 / FE65 antibody for Western blot and Mouse
Neuroscience antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes APBB1 / FE65 |
|---|---|
| Tested Reactivity | Ms |
| Predict Reactivity | Hu |
| Tested Application | WB |
| Specificity | This antibody is expected to recognise both reported isoforms (NP_001155.1 and NP_663722.1). |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | APBB1 / FE65 |
| Antigen Species | Human |
| Immunogen | C-GSLKPKRLGAHTP |
| Conjugation | Un-conjugated |
| Alternate Names | FE65; Amyloid beta A4 precursor protein-binding family B member 1; Protein Fe65; RIR; MGC:9072 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9QXJ1 Mouse Amyloid beta A4 precursor protein-binding family B member 1 |
|---|---|
| Background | The protein encoded by this gene is a member of the Fe65 protein family. It is an adaptor protein localized in the nucleus. It interacts with the Alzheimer's disease amyloid precursor protein (APP), transcription factor CP2/LSF/LBP1 and the low-density lipoprotein receptor-related protein. APP functions as a cytosolic anchoring site that can prevent the gene product's nuclear translocation. This encoded protein could play an important role in the pathogenesis of Alzheimer's disease. It is thought to regulate transcription. Also it is observed to block cell cycle progression by downregulating thymidylate synthase expression. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Mar 2012] |
| Research Area | Neuroscience antibody; Signaling Transduction antibody |
| Calculated MW | 77 kDa |
| PTM | Phosphorylation at Ser-610 by SGK1 promotes its localization to the nucleus (By similarity). Phosphorylated following nuclear translocation. Phosphorylation at Tyr-547 by ABL1 enhances transcriptional activation activity and reduces the affinity for RASD1/DEXRAS1. |
Images (1) Click the Picture to Zoom In
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ARG64035 anti-APBB1 / FE65 antibody WB image
Western Blot: NIH/3T3 lysate (35 µg protein in RIPA buffer) stained with ARG64035 anti-APBB1 / FE65 antibody at 0.1 µg/ml dilution.
