ARG45597
anti-AP2M1 antibody
anti-AP2M1 antibody for Flow cytometry,ICC/IF,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes AP2M1 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | AP2M1 |
| Antigen Species | Human |
| Immunogen | Recombinant protein containing to human AP2M1. |
| Conjugation | Un-conjugated |
| Alternate Names | AP2M1; adaptor-related protein complex 2, mu 1 subunit; Adaptor protein complex AP-2 subunit mu; Clathrin coat assembly protein AP50; Plasma membrane adaptor AP-2 50 kDa protein; Adaptin-mu2; mu2; AP-2 mu chain; Clathrin assembly protein complex 2 mu medium chain; AP-2 complex subunit mu; AP50; HA2 50 kDa subunit; Clathrin coat-associated protein AP50; Adaptor-related protein complex 2 subunit mu; CLAPM1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Observed Size | 50 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | AP2M1 |
| Gene Full Name | adaptor-related protein complex 2, mu 1 subunit |
| Background | This gene encodes a subunit of the heterotetrameric coat assembly protein complex 2 (AP2), which belongs to the adaptor complexes medium subunits family. The encoded protein is required for the activity of a vacuolar ATPase, which is responsible for proton pumping occurring in the acidification of endosomes and lysosomes. The encoded protein may also play an important role in regulating the intracellular trafficking and function of CTLA-4 protein. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2015] |
| Function | Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 mu subunit binds to transmembrane cargo proteins; it recognizes the Y-X-X-Phi motifs. The surface region interacting with to the Y-X-X-Phi motif is inaccessible in cytosolic AP-2, but becomes accessible through a conformational change following phosphorylation of AP-2 mu subunit at 'Tyr-156' in membrane-associated AP-2. The membrane-specific phosphorylation event appears to involve assembled clathrin which activates the AP-2 mu kinase AAK1 (By similarity). Plays a role in endocytosis of frizzled family members upon Wnt signaling (By similarity). [UniProt] |
| Cellular Localization | Cell membrane, Membrane, coated pit, Peripheral membrane protein, Cytoplasmic side. [UniProt] |
| Calculated MW | 50 kDa |
| PTM | Phosphoprotein. [UniProt] |
Images (5) Click the Picture to Zoom In
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ARG45597 anti-AP2M1 antibody ICC/IF image
Immunofluorescence: MCF-7 stained with ARG45597 anti-AP2M1 antibody at 5 μg/ml dilution.
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ARG45597 anti-AP2M1 antibody WB image
Western blot: HepG2 stained with ARG45597 anti-AP2M1 antibody at 0.5 μg/ml dilution.
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ARG45597 anti-AP2M1 antibody FACS image
Flow Cytometry: MCF-7 stained with ARG45597 anti-AP2M1 antibody at 1 µg/10^6 cells dilution.
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ARG45597 anti-AP2M1 antibody WB image
Western blot: Rat brain stained with ARG45597 anti-AP2M1 antibody at 0.5 μg/ml dilution.
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ARG45597 anti-AP2M1 antibody WB image
Western blot: Mouse brain stained with ARG45597 anti-AP2M1 antibody at 0.5 μg/ml dilution.
