ARG40221

anti-AMACR / p504S antibody

anti-AMACR / p504S antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Overview

Product Description Goat Polyclonal antibody recognizes AMACR / p504S
Tested Reactivity Hu
Tested Application IHC-P, WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name AMACR / p504S
Antigen Species Human
Immunogen Synthetic peptide around the internal region of Human AMACR / p504S. (C-RGSFITSEEQDVSPR. NP_055139.4; NP_001161067.1)
Conjugation Un-conjugated
Alternate Names AMACR; Alpha-Methylacyl-CoA Racemase; P504S; RACE; 2-Methylacyl-CoA Racemase; EC 5.1.99.4; AMACRD; CBAS4; RM

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P5 µg/ml
WB0.1 - 0.3 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size ~ 45 kDa

Properties

Form Liquid
Purification Ammonium sulphate precipitation followed by affinity purification with immunogen.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA.
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 23600 Human AMACR

Swiss-port # Q9UHK6 Human Alpha-methylacyl-CoA racemase

Gene Symbol AMACR
Gene Full Name alpha-methylacyl-CoA racemase
Background This gene encodes a racemase. The encoded enzyme interconverts pristanoyl-CoA and C27-bile acylCoAs between their (R)- and (S)-stereoisomers. The conversion to the (S)-stereoisomers is necessary for degradation of these substrates by peroxisomal beta-oxidation. Encoded proteins from this locus localize to both mitochondria and peroxisomes. Mutations in this gene may be associated with adult-onset sensorimotor neuropathy, pigmentary retinopathy, and adrenomyeloneuropathy due to defects in bile acid synthesis. Alternatively spliced transcript variants have been described. Read-through transcription also exists between this gene and the upstream neighboring C1QTNF3 (C1q and tumor necrosis factor related protein 3) gene.
Function Acts only on coenzyme A thioesters, not on free fatty acids, and accepts as substrates a wide range of alpha-methylacyl-CoAs, including pristanoyl-CoA, trihydroxycoprostanoyl-CoA (an intermediate in bile acid synthesis), and arylpropionic acids like the anti-inflammatory drug ibuprofen (2-(4-isobutylphenyl)propionic acid) but neither 3-methyl-branched nor linear-chain acyl-CoAs.
Cellular Localization Mitochondrion, Peroxisome
Calculated MW 42 kDa
PTM Acetylation

Images (2) Click the Picture to Zoom In

  • ARG40221 anti-AMACR / p504S antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human kidney tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG40221 anti-AMACR / p504S antibody at 5 µg/ml dilution followed by AP-staining.

  • ARG40221 anti-AMACR / p504S antibody WB image

    Western blot: 35 µg of Human kidney lysate (in RIPA buffer) stained with ARG40221 anti-AMACR / p504S antibody at 0.1 µg/ml dilution and incubated at RT for 1 hour.